Lecture 33

Question 1

DNA strand that serves as template for RNA synthesis

Answer 1

template strand

Question 2

DNA strand tha is identical in base sequence to transcribed RNA, with U in RNA in place of T in DNA

Answer 2

nontemplate strand

Question 3

another name for contemplate strand

Answer 3

coding strand

Question 4

what is DNA template strand complimentary to

Answer 4

DNA coding strand

Question 5

how many segments of bacterial RNA polymerase

Answer 5

2

Question 6

directs RNA polymerase to the correct site

Answer 6

sigma 70 subunit

Question 7

during elongation, what part of bacterial RNA polymerase dissociates

Answer 7

sigma 70 subunit

Question 8

how many channels of bacterial RNA polymerase

Answer 8

3

Question 9

NTP channel entry, what purpose

Answer 9

nucleotides come in

Question 10

what does eukaryotic RNA polymerase not have

Answer 10

sigma subunit

Question 11

RNA polymerase I synthesizes wha type of RNA

Answer 11

pre ribosomal RNA

Question 12

RNA polymerase ii synthesizes what kinds of RNA

Answer 12

mRNA, non coding

Question 13

RNA poly 3 synthesizes what kinds of RNA

Answer 13

tRNA, non coding

Question 14

proteins that bind promoter sequences to regulate transcription

Answer 14

transcription factors

Question 15

carboxyl terminal domain of RNA pol 2

Answer 15

CTD

Question 16

what polymerase is CTD related to

Answer 16

RNA poly 2

Question 17

3 bp upstream from where transcription is initiated

Answer 17

TATA box

Question 18

difference between eukaryotic transcription vs bacterial

Answer 18

lots more proteins involved in eukaryotic

Question 19

CTD is important for what

Answer 19

elongation and termination

Question 20

alpha amanitin does what

Answer 20

blocks polymerase 2, can’t transcribe DNA

Question 21

protects from premature degradation

Answer 21

5 ‘ cap

Question 22

5’ cap is what

Answer 22

7-methyl guanosine

Question 23

cuts out intron via mRNA

Answer 23

intron splicing

Question 24

what protein helps with intron splicing

Answer 24

spliceosome

Question 25

spliceosome helps with what

Answer 25

intron splicing

Question 26

3’ poly A tail added for protection, called what

Answer 26

polyadenylation

Question 27

catalyzes the synthesis of the poly A tail

Answer 27

polyadenylate polymerase

Question 28

after mRNA processing is complete, the final mature mRNA is longer or shorter than the full gene coded in DNA s

Answer 28

smaller

Question 29

using different parts of DNA for different parts of the body

Answer 29

alternative splicing

Question 30

rRNA processing occurs in what

Answer 30

bacteria

Question 31

do bacterial tRNAs contain introns

Answer 31

no

Question 32

what happens during tRNA processing

Answer 32

introns spliced, base modifications made

Question 33

noncoding RNAs include…

Answer 33

miRNA, lncRNAs, siRNAs

Question 34

function to bind mRNA and either cause degradation or inhibit translation

Answer 34

noncoding RNAs

Question 35

allows certain parts to be silenced after transcription

Answer 35

post transcription regulation