Lecture 33 Flashcards
DNA strand that serves as template for RNA synthesis
template strand
DNA strand tha is identical in base sequence to transcribed RNA, with U in RNA in place of T in DNA
nontemplate strand
another name for contemplate strand
coding strand
what is DNA template strand complimentary to
DNA coding strand
how many segments of bacterial RNA polymerase
2
directs RNA polymerase to the correct site
sigma 70 subunit
during elongation, what part of bacterial RNA polymerase dissociates
sigma 70 subunit
how many channels of bacterial RNA polymerase
3
NTP channel entry, what purpose
nucleotides come in
what does eukaryotic RNA polymerase not have
sigma subunit
RNA polymerase I synthesizes wha type of RNA
pre ribosomal RNA
RNA polymerase ii synthesizes what kinds of RNA
mRNA, non coding
RNA poly 3 synthesizes what kinds of RNA
tRNA, non coding
proteins that bind promoter sequences to regulate transcription
transcription factors
carboxyl terminal domain of RNA pol 2
CTD
what polymerase is CTD related to
RNA poly 2
3 bp upstream from where transcription is initiated
TATA box
difference between eukaryotic transcription vs bacterial
lots more proteins involved in eukaryotic
CTD is important for what
elongation and termination
alpha amanitin does what
blocks polymerase 2, can’t transcribe DNA
protects from premature degradation
5 ‘ cap
5’ cap is what
7-methyl guanosine
cuts out intron via mRNA
intron splicing
what protein helps with intron splicing
spliceosome
spliceosome helps with what
intron splicing
3’ poly A tail added for protection, called what
polyadenylation
catalyzes the synthesis of the poly A tail
polyadenylate polymerase
after mRNA processing is complete, the final mature mRNA is longer or shorter than the full gene coded in DNA s
smaller
using different parts of DNA for different parts of the body
alternative splicing
rRNA processing occurs in what
bacteria
do bacterial tRNAs contain introns
no
what happens during tRNA processing
introns spliced, base modifications made
noncoding RNAs include…
miRNA, lncRNAs, siRNAs
function to bind mRNA and either cause degradation or inhibit translation
noncoding RNAs
allows certain parts to be silenced after transcription
post transcription regulation
