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Cell Biology > Lecture 3 Microscopy > Flashcards

Lecture 3 Microscopy Flashcards

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1
Q

what is light vs electron microscopy

A

Light- shining photons

Electron- electrons on specimen

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2
Q

cells are relatively easy to see because they are _____

A

thin

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3
Q

what is a microtome

A

a machine that makes thin slices

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4
Q

Light Microscopy: light source

A

halogen

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5
Q

Light Microscopy: condenser

A

focuses light on the specimen

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6
Q

Light Microscopy: specimen

A

light goes through the specimen

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7
Q

Light Microscopy: objective

A

focuses and magnifies light from the specimen

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8
Q

Light Microscopy: ocular

A

lens that you look through

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9
Q

How do you make a bright field better

A

stain, phase contrast, DIC (differential interference microscopy)

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10
Q

what is phase contrast

A

uses diffraction rings in the condenser and objective

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11
Q

what is DIC

A

it polarizes light before and after the specimen

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12
Q

what is fluorophore

A

absorbs one wavelength of light and emits another

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13
Q

Fluorescence Microscopy: Light source

A

mercury lamp

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14
Q

Fluorescence Microscopy: excitation filter

A

allows only a specific wavelength go through

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15
Q

Fluorescence Microscopy: dischroic

A

reflects excitation light onto the specimen

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16
Q

Fluorescence Microscopy: sample

A

flurorophore absorbs excitation light and reflects back emission light

17
Q

Fluorescence Microscopy: epifluorescence

A

looking at light from the same side as the excitation light

18
Q

Fluorescence Microscopy: dichroic (2nd time)

A

allow emission light from the same side as the excitation light

19
Q

Fluorescence Microscopy: emission folter

A

allows only emission wavelengths through

20
Q

what is immunofluorescence

A

uses a fluorescent antibody to localize a specific wavelength

21
Q

what is GFP-tagging

A

green fluorescent protein which is attached to a particular protein to show where it localizes

22
Q

what is FRET

A

used to study if two proteins are next to each other

23
Q

what is FISH

A

used to show where specific DNA sequences are in a chromosome

24
Q

what is FRAP

A

used to study rate of lateral movement

25
Q

what is immunogold

A

it uses antibodies attached to gold particles

26
Q

what is tomography

A

taking images of several layers and then constructing a 3D image (CAT scan)

27
Q

what is a negative stain EM

A

it involves submerging the sample in electron dense liquid

Cell Biology (27 decks)

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