Lecture 14 Protein Sorting After the ER Flashcards
what is the secretory pathway?
ER to the Golgi then to Extracellular
What are the functions of the Golgi?
trafficking
glycosylation of proteins (adds o-link and modifies n-link)
sphingolipid synthesis
Where is cis-golgi and where is trans-golgi?
cis-golgi is the part near the ER
trans-golgi is the part near the plasma membrane
what is the cisternae
the golgi layer
What is cisternal progression?
new cisternae that is made on the cis-side
What is Anterograde/retrograde
movement along/against secretory pathway
What is vesicle fission/fusion
vesicles leaving/joining the membrane
Why is retrograde important one might say
because it keeps specific proteins or lipids from being secreted
What do COP-I, COP-II, and Clatherin do?
Make “cages” around vesicles during fission
They help pinch off membranes and “cluster” cargos as well
COP-I: retrograde vesicles
COP-II: ER to Golgi
Clatherin: Golgi to Endosome or Plasma Membrane
ARF!!!!! what is ARF?
small GTPases that regulate coat assembly
What are v-SNAREs and t-SNAREs?
proteins on vesicle
proteins on target
They squeeze membranes together for fusion
What are Rab proteins?
small GTP-ases help match t-SNAREs and v-SNAREs
what is the secretory pathway?
ER to the Golgi then to Extracellular
What are the functions of the Golgi?
trafficking
glycosylation of proteins (adds o-link and modifies n-link)
sphingolipid synthesis
Where is cis-golgi and where is trans-golgi?
cis-golgi is the part near the ER
trans-golgi is the part near the plasma membrane
