KA2- DNA replication Flashcards

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1
Q

Where does DNA replication occur?

A

The nucleus

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2
Q

When does DNA replication happen?

A

Before nuclear division

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3
Q

What does DNA replication ensure?

A

That a complete set of genetic information is passed on to new cells and the next generation

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4
Q

What does DNA replication produce?

A

Exact copies of the parent DNA molecule

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5
Q

Why is DNA unique?

A

As it can replicate itself

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6
Q

What is the first stage of DNA replication?

A

DNA unwinding

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7
Q

What does DNA unwinding create?

A

A replication fork with two template strands

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8
Q

What happens to the hydrogen bonds during first stage of DNA replication?

A

They break

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9
Q

What does a primer bind to?

A

The template strand with an exposed 3’ end

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10
Q

Why must a primer be present?

A

As DNA polymerase can only affect nucleotides to an existing strand

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11
Q

What is a primer made of?

A

A short sequence of nucleotides

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12
Q

What replication is continuous?

A

The template strand that has the 3’ end

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13
Q

What replication strand forms the leading strand?

A

The one that has continuous replication

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14
Q

What is DNA polymerase?

A

The enzyme that adds individual nucleotides to the new DNA strand

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15
Q

How do free nucleotides line up?

A

Using complementary base pairing

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16
Q

What can DNA polymerase do?

A

Only add nucleotides to the 3’ end of a primer

17
Q

What does DNA polymerase join?

A

The sugar of the primer to the phosphate of the first nucleotide

18
Q

What does DNA polymerase help form?

A

A new strand

19
Q

How does DNA polymerase continue adding nucleotides to the 3’ end of the growing strand?

A

Forming bonds between their sugars and phosphates

20
Q

Why is the leading strand continuous?

A

As the process of DNA polymerase adding nucleotides to the 3’ end is repeated until the entire molecule has been replicated

21
Q

What must happen to each fragment in the lagging strand?

A

It must be primed

22
Q

Why must each fragment be primed?

A

To allow DNA polymerase to add nucleotides

23
Q

How can DNA only grow?

A

By adding nucleotides to its 3’ end

24
Q

What type of replication is the lagging strand?

A

Discontinuous

25
Q

What happens once replication of lagging strand is complete?

A

The primer is replaced with DNA

26
Q

What does DNA ligase do?

A

Joins the fragments together to create the lagging strand of DNA

27
Q

What happens as DNA unzips?

A

More fragments will be made and connected to previous ones

28
Q

Why are replication bubbles made?

A

When a long chromosome identifies being replicated, many replication forks operate simultaneously for rapid replication it creates replication bubbles

29
Q

What are the 5 requirements for DNA replication?

A
  1. DNA
  2. Primers
  3. DNA nucleotides
  4. Enzymes
  5. ATP
30
Q

Why do we need ATP?

A

For energy

31
Q

What are the enzymes we use in DNA replication?

A

DNA polymerase
DNA ligase

32
Q

Why do we need primers?

A

Act as an existing chain for DNA polymerase to attach to

33
Q

What is DNA replication essential for?

A

The continuation of life

34
Q

What is DNA replication essential to ensure?

A

That exact copies of a specific genetic information is passed from cell to cell during growth, and from one generation till the next during reproduction