Haematology 3 - Coagulation Flashcards

1
Q

Recall 4 endogenous anti-coagulants

A

Anti-thrombin
TFPI
Protein C
Protein S

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Recalll 3 procoagulant factors and 1 anticoagulant factor that are synthesised in vascular endothelium

A

PGI2
vWF
Plasminogen activators
Thrombomodulin (anticoagulant)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Recall the 2 possible mechanisms of platelet activation

A
  1. INDIRECT - vWF binds to Gp1b which binds to platelets to activate them
  2. DIRECT - Endothelial Gp1a binds directly to the platelet to activate
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Via which receptors to platelets adhere to each other, and what is needed for this to happen?

A

GpIIb/IIIa using fibrinogen and calcium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Recall one inducer and one inhibitor of platelet aggregation, and the enzyme required for the synthesis of both

A

Thromboxane A2 increases aggregation
PGI2 inhibits platelet aggregation

COX enzyme

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Why does aspirin have an anticoagulant effect?

A

Inhibits COX enzyme which is necessary for thromboxane A2 production

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the rate-limiting step in fibrin formation?

A

Factor Xa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Which clotting factors are vitamin K dependent?

A

II, VII, IX and X (made in liver)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

How does vitamin K activate clotting factors?

A

vitamin K is required as a co-enzyme for the gamma-carboxylation of the clotting factors

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Recall 4 factors that promote fibrinolysis

A

Factor IXa, Xa, TPA and urokinase

These all increase plasmin production which cleaves fibrin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the role of plasmin?

A

Breaks down fibrin in fibrinolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the mechanism of action of heparin?

A

Augments anti-thrombin effect

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is the role of proteins C and S?

A

Inactivate Factors 5 and 8

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are the roles of tissue factor and TFPI

A

Tissue factor activates factor Xa

TFPI neutralises tissue factor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

How can platelet and coagulation factor disorders be distinguisghed clinically?

A

Platelet problems –> immediate superficial bleeding

Coagulation factor deficiencies –> delayed, deep bleeding and haemarthroses

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Recall 4 possible causes of ITP

A

Vancomycin
SLE
Sarcoidosis
Lymphoproliferative disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Why does DIC cause thrombocytopaenia?

A

Increased utilisation

18
Q

How does the presentation of auto-immune thrombocytopaenia differ between children and adults?

A

In children it tends to be acute whereas it is chronic in adults

19
Q

How should autoimmune ITP be treated?

A
Steroids
IV Ig (to compete with auto-antibody)
20
Q

What is the expected APTT and PT results in haemophilia?

A

APTT prolonged

Normal PT

21
Q

What is the inheritance pattern of von willebrand disease?

A

Autosomal dominant

22
Q

What are the 3 different types of von willebrand disease?

A

Type 1: low levels
Type 2: deficiency in function
Type 3: absent VWF

23
Q

Recall some causes of DIC

A
Sepsis
Trauma
Cancer
Obstetric complications
Vascular disorders
Reaction to a toxin
24
Q

What is DIC?

A

consumption of clotting factors and fibrin, resulting in uncontrollable bleeding

25
Q

What is the antidote to heparin?

A

Protamine sulphate

26
Q

outline the blood test results of DIC

A

elevated INR, APTT, PT, D Dimer and Bleeding time.

decrease in platelet count, fibrinogen and clotting factor levels.

27
Q

What description is given to red blood cells which are typically polychromatic and stain heavily for the presence of RNA?

A

reticulocyte

28
Q

what is heparin induced thrombocytopenia?

A

thrombocytopenia caused by antibodies directed against heparin AND platelet factor 4.
despite the low platelets it is a prothrombotic condition.

29
Q

function of thrombomodulin

A

leads to activation of Protein C

30
Q

what’s the difference between aspirin and NSAIDs in terms of mechanism

A

aspirin irreversibly inhibit COX
NSAIDs inhibit reversibly

in the archidonic acid pathway

31
Q

4 effects of thrombin

A

o Activates fibrinogen
o Activates platelets
o Activates pro-cofactors (Factor 5 and Factor 8)
o Activates zymogens (Factor 7, 11 and 13)

32
Q

how does bile affect vitamin K

A

Vitamin K is fat soluble so need bile to absorb vitamin K (i.e. bile duct obstruction causes deficiency)

33
Q

how do bacteria affect vitamin K

A

bacteria help make Vit K so, antibiotics can harm gut flora reaching Vit K absorption

34
Q

most common cause of vitamin A deficiency

A

warfarin

35
Q

2 inhibitors of plasmin

A

Alpha-2 antiplasmin

Alpha-2 macroglobulin

36
Q

give 2 distinct roles of thrombin

A

as a procoagulant when it converts fibrinogen into an insoluble fibrin clot

thrombin activates protein C, it acts as an anticoagulant.

37
Q

how many antithrombins are there? which is most potent

A

five in total

most potent = AT 3 potentiator = heparin

38
Q

what is the most thrombogenic condition

A

lack or deficiency of antithrombin

39
Q

state 3 physiological anticoagulants & their mechanisms

A

antithrombin - Inhibits thrombin,
protein C & S - inactivates F5a and F8a
tissue factor pathway inhibitor - inactivates 7a

40
Q

drug to reduce warfare overdose

A

PCC

prothrombinase complex concentrate (contains vit K dep clottting factors)