GPCR Flashcards

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1
Q

Role of GTP

A

provides energy for the signal

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2
Q

purpose of GPCR

A

detect extracellular signal & transduce it into an intercellular response

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3
Q

STRUCTURE of GPCR

A

N terminal
extracellular domain
7 transmembrane domains
C terminus of protein: heterotrimeric- 3 subunits

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4
Q

what happens at N terminal of GPCR?

A

chemical binds to it
conformational change within receptor complex which has an impact on C terminal

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5
Q

G protein activation

A

signalling molecule binds to the GPCR and G-protein alpha subunit exchanges GDP for GTP
alpha subunit dissociates from beta and gamma subunits and triggers a cellular response
GTP is hydrolysed to GDP and signalling molecule comes off
alpha subunit comes back together with the receptor and the beta and gamma subunits

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6
Q

signalling pathway for G alpha S

A

stimulates:
Adenylate (adenylyl) cyclase converts ATP into cyclic AMP
cAMP is a key second messenger in cells
activates protein kinase A, phosphorylation of target proteins

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7
Q

signalling pathway for G alpha I

A

inhibits: Adenylate (adenylyl) cyclase which converts ATP into cyclic AMP

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8
Q

signalling pathway for G alpha q

A

activates phospholipase C
PLC breaks PIP2 down (hydolyzes) to IP3 & DAG (key second messengers)
IP3- causes release of Ca2+
DAG- activates protein kinase C

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9
Q

what is PIP2?

A

membrane phospholipid

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10
Q

IP3 CAUSES?

A

release of Ca2+

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11
Q

DAG activates?

A

protein kinase C

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