EX. (Recoding) Flashcards
Use to differentiate microorganisms to utilize of carbohydrates
Triple sugar iron
What are the carbohydrates seen in the slant of triple sugar iron
Sucrose and lactose
What are the carbohydrates seen in the Butt of triple sugar iron
Glucose
What bacteria are lactose fermenter?
Klebsiella sp.
Escherichia coli
Enterbacteriaceae
Zone of inhibition (urine culture)
15-17 - sus
<15 - Res
in between - intermediate
Example of parasite with flagella?
Giardia lamblia
Example of parasite with pseudopodia
Entamoeba histolytica
What test use to identify fungal infection?
Potassium hydroxide test
What is the order of bacteria in classification (Ecoli)
D - Bacteria K - None P - Proteobacteria C - Gamma proteobacteria O - Enterobacteriales F - Enterobacteriaceae G - Escherichia S - E. coli
If MRSA is resistant to Vancomycin and penicillin, what is the net agent?
Clindamycin
Leading cause of Urinary tract infection
Escherichia coli
Best to detect MRSA?
Cefoxitin
Drug of choice for UTI with E. coli?
Nitrofurantoin antibiotic
Other name of Mycobacterium tuberculosis
Koch bacilli
Species with different susceptibility patterns
Strain
Composed of polysaccharide (Somatic or O antigen) useful
for distinguishing serovars. i.e. E. coli 0157:H7.
O-specific-polysaccharide
What is the characteristics of capsulated bacteria in BAP?
Mucoid colony
A staining that targets the background to make microorganisms to appear
Negative stain
What infection cause by S. pneumoniae?
Meningitis and lumbar pneumonia
What can be seen in BAP if there is swarming motility
Proteus
Fimbriae is also known as ?
Common pili
Fence like or chinese character shape
Palisade
How to detect spirochetes?
Dark-Field microscope
What to do before gram staining?
Air dried then heat fix
It intensifies primary stain
Mordant
In acid fast stain, zeil neelsen, how many minutes to heat before washing?
10 mins
Smearing in sputum that there is an actual size and proper way to apply the sample to achieve eveness
Coiling
It is the certain area of the slide that we can analyze?
Zone of morphology
What targets acridine orange?
Nucleic acid
What agar use in capnophilic bacteria?
Chocolate agar
In direct plate count, what equipment is used?
Nephelometer and spectro
Less than 50,000 in urinary culture
contaminant
50,000 - 100000 in urinary culture
Significant growth
100,000> in urinary culture
Clinically significant colony count
What is the name of sodium citrate in CMA utilization?
Simmon’s citrate (Slant media)
What bacteria is positive in simmon’s citrate?
Enterobacteriaceae
K. pneumoniae
Citrobacter pneumoniae
What is the positive indicator for simmon’s citrate?
Blue
Most common use for fermentation
Triple sugar iron
Another name of krebs cycle
(Tricarboxylic acid cycle)
In lactose fermentation what is the positive indicator in MacConkey agar?
Pink: Beta galactoside Permeases
Breaks down glucose to galactose
Beta galactosidase
