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Bacte Lec (Overview) [Prelims 1] > 4.4.4 Laboratory Diagnosis > Flashcards

4.4.4 Laboratory Diagnosis Flashcards

1
Q

What is the Microscopical characteristics of bacillus anthracis

A

Large
Gram + rod
Square ended
Oval spores
Non-motile

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2
Q

Spores of B. anthracis is located?

A

Centrally to subterminally

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3
Q

What is the appearance of B. anthracis in the microscope?

A

Bamboo rod

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4
Q

What it the cultural characteristics of B. anthracis?

A

Non-hemolytic
Large
Gray
Flat
Irregular margin (Medusa Head)

Beaten egg white consistency

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5
Q

What is the cultural characteristics of B. anthracis in Bicarbonate agar?

A

Large
Mucoid

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6
Q

What is the cultural characteristics of B. anthracis in Mueller-Hinton agar (Contains 0.05-0.5 U/mL penicillin)?

A

String of pearls

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7
Q

What is the microscopic characteristics of B. cereus?

A

Large
Oval spores (Centrally - Subterminally)
Bacilli
Motile

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8
Q

What is colony appearance of B. cereus?

A

Large
Feathery
Spreading
Beta-hemolysis

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9
Q

What is the main difference between B. anthracis and B. cereus?

A

Beta hemolytic (Cereus)

Motility

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10
Q

Test use to differentiate Clinically isolated bacillus spp.

A

Lecithinase test

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11
Q

What is the positive indicator for lecithinase test?

A

Opaque zone of precipitation (Surrounds the colonies)

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12
Q

What are the Bacillus spp. positive in Lecithinase test?

A

anthracis
cereus
thuringiensis
mycoides

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13
Q

Test to differentiate between B. cereus and B. anthracis

A

Motility test (Semisolid agar)

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14
Q

What is the positive indicator for semisolid agar?

A

spreading of organisms out from the site of inoculation

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15
Q

What is the Negative indicator for semisolid agar?

A

Remain at the site

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16
Q

What organism is positive in motility test?

A

B. cereus
B. thuringiensis

17
Q

What organism is negative in motility test?

A

B. anthracis
B. mycoides

18
Q

What organism is susceptible in penicillin susceptibility test

A

Bacillus anthracis

19
Q

What is use to treat bacillus spp.?

A

Ciprofloxacin

Bacte Lec (Overview) [Prelims 1] (34 decks)

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