5.4.1 Gardnarella vaginalis Flashcards
What is the characteristics of Gardnerella vaginalis?
Short
Coccobacillus
What reagent inhibits Gardnerella vaginalis
Sodium polyanethol sulfonate
What is the normal flora for G. vaginalis
Vaginal tissue
What is the clinical infection for G. vaginalis?
Bacterial vaginosis
What happens to the pH in Bacterial vaginosis?
> 4.5
Less commonly associated UTI
Bacterial vaginosis
What is the normal pH for vagina?
3.8-4.5
What other bacteria causes bacterial vaginosis?
Chlamydia trachomatis
Trichomonas vaginalis
What is the smell of discharge in bacterial vaginosis?
Fishy odor
What is the microscopic characteristics of G. vaginalis?
Pleomorphic
Gram variable
Coccobacillus
“Clue cells” in wet mount
What is the description of clue cell in G. vaginalis?
Sunny side up
What is the cultural characteristics f G. vaginalis
Pinpoint
Non-hemolytic (BAP)
Beta hemolytic in human Blood bilayer tween (HBT) agar
What is supplemented in HBT agar?
Tween 80
What is the media of choice for HBT?
(On Lower part of the base)
Colistin Nalidixic acid (CNA)
Amphotericin B
What is the media of choice for HBT?
(On Upper part of the base)
5% human blood
Enhances the production of Beta hemolysis of G. vaginalis?
Located on both bilayer
Tween agar
What is the presumptive test for G. vaginalis?
Whiff test
What do you do in whiff test
Smell the fish odor
What is being added in whiff test before whiffing it
1 drop of 10% KOH to the vaginal washing
What does KOH do in whiff test?
Releases the amine (Fish odor)
What are the components of amines?
Putrescine
Cadaverine
histamine
What is the treatment for G. vaginalis?
Metronidazole
