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Bacte Lec (Overview) [Prelims 1] > 2.2.2 Biochemical test (Gram positive, Catalase Negative) > Flashcards

2.2.2 Biochemical test (Gram positive, Catalase Negative) Flashcards

1
Q

A test that is use to differentiate Staph and micrococcus but can also used to differentiate S. pyogenes

A

Bacitracin

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2
Q

In bacitracin, who is susceptible? (Gram pos, Cat neg)

A

S. pyogenes

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3
Q

In bacitracin, who is Resistant? (Gram pos, Cat neg)

A

Other strept. except pyogenes

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4
Q

Positive indicator for Bacitracin?

A

10mm zone of inhibition

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5
Q

A test that is use to differentiate Beta hemolytic streptococcal

A

Sulfamethoxazole-Trimethoprim (SXT)

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6
Q

What is the Susceptible in SXT?

A

Non “beta” hemolytic Streptococcaceae

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7
Q

What is the Resistant for SXT?

A

S. pyogenes
S. agalactiae
S. dysagalactiae subsp. equisimilis

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8
Q

How many grams for SXT?

A

1.25ug

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9
Q

Test for the ability of the organism to hydrolyze the substrate L-pyrrolidonyl-β-napththylamide (PYR).

A

Pyrrolidonyl aminopeptidase / Pyrrolidonyl-beta-naphthylamide

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10
Q

What bacteria is positive in PYR?

A

Group A
Group D Enterococcus

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11
Q

What bacteria is Negative in PYR?

A

The rest
Group D non enterococcus

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12
Q

Positive indicator for PYR?

A

5 mins Bright red (pink to cherry red)

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13
Q

A test used for the presumptive identification of group B streptococci (S.
agalactiae), Clostridium, Listeria

A

CAMP (Christie-Atkins-Munch-Peterson)

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14
Q

Test for synergistic hemolysis between group B Streptococcus and βhemolytic Staph. aureus

A

CAMP (Christie-Atkins-Munch-Peterson)

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15
Q

In CAMP, what bacteria is swabbed vertically?

A

Staphylococcus aureus

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16
Q

In CAMP, what bacteria is swabbed horizontally?

A

Group B strept
Listeria monocytogenes

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17
Q

What is the positive indicator for CAMP?

A

Arrowhead

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18
Q

In CAMP, what is being detected in Group B Strept?

A

CAMP factor

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19
Q

In CAMP, if Clostridium is being identified, what bacteria is vertical?

A

S. agalactiae

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20
Q

What is the positive indicator for Clostridium in CAMP?

A

Reversed arrowhead / Bowtie

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21
Q

What is the positive indicator for L. monocytogenes in CAMP?

A

Black arrowhead

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22
Q

It is test for hippuricase enzyme of Group B Streptococci

A

Hippurate hydrolysis

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23
Q

IT hydrolyzes sodium hippurate to sodium citrate and Ninhydrin

A

Hippuricase enzyme

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24
Q

What are the reagents of Hippurate hydrolysis?

A

Sodium hippurate and ninhydrine

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25
Q

What is the positive indicator for Hippurate hydrolysis

A

Deep blue color

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26
Q

What microorganisms is positive in hippurate hydrolysis?

A

S. agalactiae

27
Q

Detects for the ability of the bacteria to hydrolyze Leucine-β-naphthylamide

A

LAP (Leucine aminopeptidase)

28
Q

What is the reagent for LAP?

A

Cinnamaldehyde

29
Q

What organisms is positive in LAP test?

A

All viridans

(S. pneumoniae and
S. pyogenes,
Enterococcus,
Pediococcus) - ppt

30
Q

What is the positive indicator for LAP?

A

Red color in 1 min

31
Q

Detects the action of β-D-glucuronidase

A

B-D-aminopeptidase

32
Q

where can β-D-glucuronidase be found in what bacteria?

A

Large colony forming Beta hemolytic group C and G

33
Q

What is the positive for B-D-aminopeptidase?

A

G, C group

34
Q

What is the Negative for B-D-aminopeptidase?

A

Group F (Strep. anginosus)

35
Q

Detects for the ability of the bacteria to grow in the presence of 40% bile and
hydrolyze esculin

A

Bile Esculin (Bile esculin hydrolysis)

36
Q

What are the reagents for bile esculin?

A

40% bile
Ferric NH4 (ammonium)

37
Q

What is the positive indicator for Bile esculin?

A

Black

38
Q

What is positive for Bile esculin?

A

ALL Group D

39
Q

Test the ability of the organism to grow in 6.5% NaCl

A

Salt tolerance test

40
Q

Differentiates Group D strep

A

Salt tolerance test

41
Q

What is the positive indicator for Salt tolerance test?

A

Turbidity and color change (After 18-24 hours)

42
Q

What is positive in Salt tolerance test?

A

Group D enterococcus

43
Q

What is Negative in Salt tolerance test?

A

Group D non-enterococcus

44
Q

Known as Taxo P (Ethylhydrocupreine hydrochloride) that differentiates S. pneumoniae from alpha hemolytic

A

Optochin Susceptibility

45
Q

how many grams of Ethylhydrocupreine hydrochloride in optochin susceptibility test?

A

5ug

46
Q

What is the Susceptible indicator for optochin susceptibility?

A

14 or 16 mm zone of inhibition

47
Q

What is the Resistant indicator for optochin susceptibility?

A

<14 or 16 mm zone of inhibition

48
Q

What is organisms is susceptible in Optochin sus?

A

S. pneumoniae

49
Q

Confirmation test in determining the alpha hemolytic

Differentiates S. pneumoniae and viridans

A

Bile solubility test

50
Q

2 methods of bile solubility test

A

BAP
Broth

51
Q

What reagent is used in bile solubility?

A

Sodium deoxycholate

52
Q

How man Percent in BAP for sodium deoxycholate?

A

10%

53
Q

How man Percent in Broth for sodium deoxycholate?

A

2%

54
Q

What is the positive indicator for BAP in Bile solubility?

A

Clear and lyse colony

55
Q

What is the negative indicator for BAP in Bile solubility?

A

Intact colony

56
Q

What is the positive indicator for broth in Bile solubility?

A

Clear

57
Q

What is the Negative indicator for broth in Bile solubility?

A

turbid

58
Q

What is soluble in Bile solubility?

A

S. pneumoniae

59
Q

What is insoluble in Bile solubility?

A

Viridans

60
Q

What are the Test for Group A?

A

Bacitracin
PYR

61
Q

What are the Test for Group B?

A

CAMP
Hippurate hydrolysis

62
Q

What are the Test for Viridans and S. pneumoniae?

A

Optochin susceptibility
Bile solubility

63
Q

What are the Test for Group D?

A

PYR
Bile esculin
Salt tolerance (6.5%)

64
Q

What are the Test for Streptococcal like organisms?

A

LAP

Bacte Lec (Overview) [Prelims 1] (34 decks)

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