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Bacte Lec (Overview) [Prelims 1] > 3.1.5 Lab Diagnosis > Flashcards

3.1.5 Lab Diagnosis Flashcards

1
Q

What are the specimen use for N. gonorrhoeae?

A

Genitalia: Urethra, cervix

Rectum and pharynx

Eye discharge

Blood/Joint fluids

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2
Q

How many cm is used to insert in the rectum?

A

4-5 cm

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3
Q

How many cm is used to insert in the Urethra?

A

2 cm while slowly rotating

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4
Q

Why you shouldn’t use cotton with fatty acid?

A

Inhibit growth of N. gonorrhoeae

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5
Q

What are the 4 swabs?

A

Dacron swab
Polyurethane swab
Rayon swab
Flocked nylon swab

(DaPoRaF)

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6
Q

What is the best swab use that does not contain fatty acid?

A

Dacron swab
Rayon swab

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7
Q

What specimen is used for Ophthalmia neonatorum

A

Eye discharge

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8
Q

Where is blood/join fluid is used?

A

Disseminated gonococcal infection

Gonococcal arthritis

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9
Q

What is the ideal specimen for N. meningitidis?

A

Cerebrospinal fluid

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10
Q

How to improve detection of CSF?

A

Increase concentration

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11
Q

What is the amount of required CSF?

A

1 mL at 1000xg for 10 mins in Centrifuge

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12
Q

What are the other specimen use for n. meningitidis?

A

Nasopharyngeal swabs and aspirates

Blood

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13
Q

What are the specimen use for M. catarrhalis?

A

Middle ear effusion
Nasopharynx
Sinus aspirates
Sputum
Bronchial aspirates

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14
Q

Cotton swabs contains a reagent that inhibits the growth of N. gonorrhoeae

A

Calcium alginate

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15
Q

What are the transport media use for Neisseria gonorrhoeae?

A

Transgrow
JEMBEC
Bio-bag
Gono-Pak
Amies medium

(BAG JT)

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16
Q

When using Amies medium within how many hours should be plated?

A

6 hours

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17
Q

What is the selective media for Neisseria sp.?

A

Chocolate agar plate

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18
Q

In direct microscopic examination, what is usually the specimen?

A

Urogenital/Eye discharge

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19
Q

What is the shaped of N. gonorrhoeae and meningitidis

A

kidney/coffee bean shape

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20
Q

The most commonly seen N. gonorrhoeae is called?

A

Intracellular gram negative diplococci

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21
Q

N. gonorrhoeae is can be seen in both intracellular and extracellular

A

TRUE

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22
Q

Moraxella catarrhalis can only be seen Intracellularly?

A

TRUE

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23
Q

What is the test for M. catarrhalis?

A

Penicillin disk test

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24
Q

REVIEW

A

If you see a gram-negative rod, it might be Kingella spp. If it’s an
elongated gram-negative rod, it is Acinetobacter spp.

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25
Q

What is the culture media of choice for Neisseria?

A

Chocolate Agar plate

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26
Q

What is the composition for thayer Martin agar?

A

VCN
(Vancomycin, Colistin, Nystatin)

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27
Q

What is the function of Vancomycin?

A

Inhibit gram positive

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28
Q

What is the function of Colistin?

A

Inhibit gram negative

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29
Q

What is the function of Nystatin?

A

Inhibit yeast cell

30
Q

What is the composition for Modified Thayer Martin agar?

A
  • VCN
  • Trimethoprim lactate
31
Q

What is the function of Trimethoprim lactate?

A

Inhibit swarming Proteus

32
Q

What is the composition for Martin Lewis agar?

A

VCT
(Vancomycin, Colistin, Trimethoprim lactate)

  • Anisomycin
33
Q

What is the function of Anisomycin?

A

Inhibits yeast cell

34
Q

What is the composition for New York City agar?

A
  • VCT
  • Amphotericin B
35
Q

What is the function of Amphotericin B?

A

Inhibits yeast

36
Q

What is the composition for GC agar?

A
  • VCT
  • Amphotericin B
  • Lincomycin
37
Q

What is the function of Lincomycin?

A

Inhibits Gram Positive

38
Q

What is the most commonly used culture media for Neisseria sp?

A

Thayer Martin

39
Q

What is the Temp in the incubation for Neisseria and how long it will be?

A

35C in 72 hrs 3-5% CO2

40
Q

Where is the Neisseria media being place before incubation?

A

Candle jar

41
Q

What type of candle jar should be used?

A

White
unscented

42
Q

What is the characteristics of N. gonorrhoeae in Agar plate?

A

Small
tan
Translucent
Raised

43
Q

If we see in the gram-stain more than 5 WBCs and/or pus cells BUT
without the presence of any bacteria AT ALL, What is this hidden bacteria?

A

Chlamydia trachomatis
Europlasma eurolyticum

44
Q

What is the characteristics of N. meningitidis in Agar plate?

A

Small
tan
mucoid
convex

45
Q

N. meningitidis can grow on both BAP and CAP?

A

TRUE

46
Q

What is the characteristics of M. catarrhalis in Agar plate?

A

Smooth
opaque
gray to white

47
Q

What are the Biochemical test for Neisseria?

A

Oxidase test
Superoxal test
Carbohydrate utilization
Dnase
Butyrate Esterase test

48
Q

Determines presence of the cytochrome oxidase system that oxidizes cytochrome with molecular oxygen

A

Oxidase test

49
Q

Where is oxidase test usually test on?

A

Filter paper
Directly on plate

50
Q

What is the main reagent of oxidase test?

A

Tetramethyl-p-phenylene-diamine-dihydrochloride

51
Q

What is the positive indicator for Oxidase test?

A

Purple / Deep purple

52
Q

What is the test that uses 30% hydrogen peroxide?

A

Superoxol catalase test

53
Q

What is the positive indicator for Superoxol catalase test?

A

Bubbles

54
Q

What only bacteria is positive on 30% hydrogen peroxide?

A

N. gonorrhoeae

55
Q

All Neisseria is positive on 3% hydrogen peroxide except?

A

elongata
bacilliformis

56
Q

What is the media for Carbohydrat Utilization test?

A

Cystine trypticase agar

57
Q

Cystine trypticase agar contains 1% of what carbs?

A

Glucose
Maltose
Lactose
Fructose
Sucrose

58
Q

Cystine trypticase agar contains carbs that are in different tubes?

A

TRUE

59
Q

What is the pH indicator for Cysteine trypticase agar?

A

Phenol red

60
Q

What is the positive indicator for Cysteine trypticase agar?

A

yellow

61
Q

How cysteine trypticase agar turns carbs into yellow?

A

In anaerobic environment, it will change to acid

62
Q

All Neisseria are GLUCOSE fermenter except?

A

flavescens
M. catarrhalis

63
Q

N. flavescens and M. catarrhalis are?

A

Asaccharolytic

  • does not breakdown sugar in anaerobic environment
64
Q

What bacteria is only positive in GLUCOSE?

A

N. gonorrhoeae

65
Q

What bacteria is only positive in
- Glucose
- Maltose

A

N. meningitidis

66
Q

What bacteria is only positive in
- Glucose
- Maltose
- LACTOSE

A

N. lactamica

67
Q

What bacteria is only positive in
- Glucose
- Maltose
- Sucrose
- Fructose

A

N. sicca

68
Q

What bacteria is positive for Dnase test?

A

M. catarrhalis

69
Q

What test that adds colony then substrate that is similar to oxidase test?

A

Butyrate Disk / Tributyrin hydrolysis

70
Q

What is the positive indicator for Butyrate disk?

A

Blue

71
Q

What bacteria is positive to Butyrate test?

A

M. catarrhalis

Bacte Lec (Overview) [Prelims 1] (34 decks)

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