4.1.1 Gram positive, Non-branching, Catalase Positive Bacilli, Introduction

Question 1

What is the the first thing to differentiate in Gram positive bacilli?

Answer 1

Spore forming and non spore forming

Question 2

Test that differentiates spore forming bacteria

Answer 2

Beta-hemolytic motile

Question 3

What bacteria is positive in Beta-hemolytic motile?

Answer 3

Bacillus spp.

Question 4

What bacteria is Negative in Beta-hemolytic motile?

Answer 4

Bacillus anthracis

Question 5

What are the non-branching bacteria that are catalase Positive?

Answer 5

Corynebacterium
Listeria

Question 6

What is branching?

Answer 6

Filamentous Microscopically
Aerobic actinomycetes
Partially Acid fast

(NO cardia)

Question 7

What is the test use to differentiate Spore forming and non spore forming bacteria

Answer 7

Lecithinase test

Question 8

What bacteria is positive in Lecithinase test?

Answer 8

Spore forming

Question 9

How many spp in Corynebacterium?

Answer 9

60 species (40 known pathogenic)

Question 10

Corynebacterium is resistant to?

Answer 10

Drying

Question 11

What is the normal flora of Corynebacterium?

Answer 11

Skin and mucous membrane

Question 12

Why Corynebacterium cell wall closely related to Nocardia?

Answer 12

Partially fast due to:
- Short chained Mycolic acid
- m-DAP (meso-diaminopimelic acid)

Question 13

Lipophilic Corynebacterium are often considered?

Answer 13

Fastidious (Complex nutrition)
Slow grower

Question 14

How many hrs does Lipophilic corynebacterium grow?

Answer 14

48-72 hours

Question 15

What is the gaseous composition for Corynebacterium?

Answer 15

Facultatively anaerobic

Question 16

What is the arrangement of Corynebacterium?

Answer 16

Palisade

Question 17

Can Corynebacterium exhibit pleomorphism? (Different shapes)

Answer 17

TRUE

Question 18

A Polymerized phosphates that is seen in metachromatic granules during methylene blue staining

Answer 18

Babes-Ernst granules