4.3.3 Lab diagnosis

Question 1

What is the microscopy characteristics of monocytogenes?

Answer 1

Gram positive
Coccobacilli / short chains

Question 2

What is the cultural characteristics of Listeria monocytogenes

Answer 2

Small
Round
Smooth
Translucent
B-hemolytic

Question 3

Listeria monocytogenes grows best at?

Answer 3

30-35

(But growth occurs at 0.5-45)

Question 4

Listeria monocytogenes can be grown on what enrichment?

Answer 4

Cold enrichment (4C)

Question 5

What is the motility for Listeria monocytogenes in wet mount?

Answer 5

End-over-end tumbling motility

Question 6

What is the pattern of Listeria monocytogenes in semi-solid tube?

Answer 6

Umbrella-shaped or inverted christmas tree pattern

Question 7

Leading cause of blood bag contamination?

Answer 7

Listeria monocytogenes

(pede si Yersinia enterocolitica)

Question 8

What is the reaction of Listeria in CAMP?

Answer 8

Block arrowhead

(Formation of rectangle block type)

Question 9

What is the reaction of Listeria monocytogenes in Hippurate hydrolysis test?

Answer 9

Deep blue

Question 10

What is the reaction of bile esculin for listeria monocytogenes

Answer 10

Black

Question 11

What are lab test that are positive in Listeria monocytogenes?

Answer 11

CAMP
Hippurate hydrolysis
Bile esculin

Question 12

What is the media use for motility test?

Answer 12

Sulfide indole motility