Dna Test Flashcards
What are the purine and pyrimidine bases
Purine- adenine guanine
Pyrimidine- thymine and cytosine
Review the picture,
What are all the parts
Shape of DNA
Double helix
The information that directs replication transcription and translation are found in what
DNA’s bases
What is chargaffs rule
Base pairing in DNA was determined from the rule, the percentage of pyrimidine a in DNA is about equal to the percentage of purines, showing the base pairings
DNA to DNA is known as
Replication
DNA to RNA is known as
Transcription
RNA to amino acid sequence is known as
Translation
Where do replication and transcription occur
Nucleus
Where does translation occur
Cytoplasm attached to ribosome
What is different between leading strand and lagging
Leading strand is replicated continuously, lagging strand is replicated discontinuous my forming Okazaki fragments
Review the mutations on review
26-28
Positions available in the genetics industry, hundreds of entry level openings for tireless workers. No previous experience needed, must be able to transcribe code in nuclear environment
RNA polymerase
Accuracy and speed vital for the job in translation, skills in transporting and positioning amino acid
tRNA
Executive position available, must maintain genetic continuity through replication and control cellular activity by regulation of enzyme production
DNA
Supervisor of production of proteins-all shifts, must be able to follow exact directions from double stranded template, travel from nucleus to the cytoplasm
mRNA
Review questions 35 and 38, 39, 41, 57
The sequence code
What parts are to the nucleotide in DNA
Deoxyribose, phosphate, + C G T A
Because of base pairing the percentage of —- in DNA is about equal to the percentage of —-
Pyrimidines= purines
DNA is copied in what process
Semi conservative replication
Difference between RNA and DNA
RNA- ribose sugar, single strand, uracil no T
DNA- deoxyribose, double strand, T no U
What types of RNA are used in protein synthesis
mRNA, rRNA, tRNA
During transcription where is RNA formed and its qualities
Inside nucleus
Single stranded
Complementary to one strands of DNA
Why is it possible for an amino acid to be specified by more than one kind of codon
There are 64 different kinds of codons but only 20 amino acids
What RNA is a blueprint of genetic code
mRNA
What happens in translation
Cel uses information from messenger RNA to produce proteins
During translation the type of amino acid that is added to polypeptide chain depends on what
The codon on mRNA and the anticodon on tRNA
Different gene mutations
Insertion, deletion, substitution, point, and frame shift
Insertion
Addition of an extra nucleotide, results in frame shift mutation
Deletion
Removal or an original nucleotide, results in frame shift mutation
Substitution
Change of a nucleotide to another, result in point mutation
Point mutation
Mutation affecting one or a few nucleotides
Frame shift mutation
Change all codons that follow the mutation
What is a promoter
Binding site for RNA polymerase
RNA polymerase
Used to transcribe RNA from DNA, regulate RNA synthesis, finds to specific section where mRNA will be synthesized. As DNA strand unwinds the poly. Initiated mRNA synthesis by moving 3-5’ along DNA strand. The DNA strand that is read by the poly. Is called template strand. Once mRNA is complete the poly detaches
What can result from DNA mutation
Cancer (from mutated body cell) decreased functioning my birth defects ( sex cell mutated) or no problem observed
What other functions can RNA perform
Catalyze F chemical reactions processing messenger RNA after transcription
Helping DNA replicate
DNA helicase
Unwinds and unzips DNA double helix during replication (breaks hydrogen)
DNA Ligase
Bunds Okazaki fragments together
DNA polymerase
Catalyze the addition of DNA nucleotides to make replicates adding to the 3’ end of the new strand
( builds the new strands of DNA from the old strand (builds the hydrogen and phosphodiester)
RNA Primase
Adds short pieces of RNA to each strand of DNA to help keep the helix open and to as a starter piece with a 3’ end
RNA polymerase
Catalyze the addition of RNA nucleotides to transcribe DNA into RNA adding to the 3’ end of new strand
Chromatin
Loosely coiled DNA (in this state during interphase)
Chromatids
Tightly coiled DNA found during mitosis
Chromosomes
Pair of identical chromatids that are joined by a centromere
How many sets of chromosomes are in the human body cell
2
How many total chromosomes are in the human body
46