DNA Replication Flashcards

1
Q

When does DNA replication occur?

A

Interphase

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2
Q

What does DNA replication produce?

A

Two exact replicas of the DNA model

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3
Q

What does the new DNA molecules look like?

A

Each new chromosome consists of two identical chromatids and are attached at the centromere

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4
Q

What bonds hold nucleotides together?

A

Hydrogen

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5
Q

What is the role of helicase?

A

Unwinds and unzips DNA

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6
Q

What are the SSBP for?

A

To stop the strands from joining and from folding over one another

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7
Q

How many template strands are there

A

2

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8
Q

What does RNA primase do?

A

Synthesises RNA primers that are complementary to the DNA template

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9
Q

What is the role of DNA polymerase III?

A

Catalyses the addition of free nucleotides complementary to the template.

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10
Q

What direction do new strands HAVE to be made in ?

A

5-3

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11
Q

What is the leading strand?

A

The Strand of DNA in which runs 5-3

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12
Q

What is the lagging strand?

A

The strand of DNA which runs 3-5

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13
Q

What happens with the lagging strand?

A

It must be built backwards from the position of the RNA primer. These short fragments are known as Okazaki fragments

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14
Q

What is the role of DNA polymerase I?

A

To remove RNA primers and replace them with DNA

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15
Q

What is the role of ligase?

A

Joins neighbouring fragments together

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16
Q

Explain semi-conservative

A

Replicated DNA will contain one old and one new strand of DNA this meaning the replication is semi conservative

17
Q

Why is DNA replication nesscary?

A

It is the first step in cell divison for both mitosis and meisos

18
Q

What is the replication fork?

A

The point in which each strand goes in either direction.

19
Q

What sort of strand is used as a template?

A

The parent strand

20
Q

How much genetic information does the new chromsome have in comparison with the original?

A

Double

21
Q

What is the lagging strand formed in?

A

Fragments

22
Q

Summarise in steps the process of cell division

A
  1. Helicase unwinds the parental DNA
  2. DNA polymerase III catalyses the addition of free nucleotides complementary to the template.
  3. Dna polymerase I cuts out RNA primer and replaces with DNA
  4. Ligase joins neighbouring fragments
23
Q

Which are the purines?

A

Adenine and Guanine

24
Q

What are the Pyrimidines

A

Thymine and Cytosine (Uracil)

25
Q

How many bonds does A-T have

A

2

26
Q

How many bonds does G-C have?

A

3

27
Q

What phase is interphase in?

A

S