DNA profiling and Gel electrophoresis

Question 1

what is DNA profiling also called

Answer 1

• DNA finger printing
• DNA testing
• DNA typing

Question 2

what is DNA profiling

Answer 2

Is a forensic technique used to comapre the base sequanenes of teo or more individuals to determine how similar they are.

Question 3

what does DNA profilling use

Answer 3

short tandem repeats

Question 4

what are short tandem repeats (STRs)

Answer 4

STRs are sections of non-coding DNA that are repeated in the DNA. They are multiple loci and are used to compare.

Question 5

what are the four steps of the genetic fingerprinting process

Answer 5

1. Extract DNA from a sample
2. STRs cut into sections using restriction enzymes
3. Amplifiied using PCR
4. Separate fragments using gel electrophoresis

Question 6

what are the five uses of DNA profiling

Answer 6

1. DNA identification
2. Genetic fingerprinting
3. Paternity tests
4. Identifying diseases
5. bloodlines of livestock

Question 7

what are the five steps of gel electrophoresis?

Answer 7

1. Restriction enzymes cut up DNA
2. A fluorescent dye is added to samples
3. Samples are added to the wells in the gel at the negative electrode end
4. Electricity is run through the gel and the negatively charged DNA is attracted to the positive electrode
5. DNA is viewed under UV light which causes it to be fluoresce