Characterizing and Classifying Prokaryotes Flashcards
Most diverse group of cellular microbes
Prokaryotes
Habitat Ranges of Prokaryotes
From Antarctic glaciers - Thermal hotsprings
Animal colons - cytoplasm of other
From distilled water - Superaturated water prokaryotes
from disinfectant solutions - Basalt rocks
Few of all prokaryotes are capable of colonizing
Human and causing disease
Coccus
Round
Coccobacillus
Oval
Bacillus
Rob
Vibrio
Curved rod
Spirillum
Stiff Spiral
All reproduce asexually
Reproducation of Prokaryotic cells
3 Main methods of reproduction of prokaryotic cells
Binary fission
Snapping divison
Budding
Binary Fission
Dna Attaches to cytoplasmic membrane
-Daughter cells may separate, or stay attached
Snapping division, a variation of binary fission
Occurs in Gram + bacteria
As new cell develops, the inner portion of the cell wall pushes on outer cell wall eventually snapping into two separate organisms
Well cells divide everything is split _______ in binary fission
50-50
Budding
- Out growth, nucleic acid transferred, but enlarges into a new cell
- The Larger parent cell can continue to produce more buds
Reproductive cells produce spores at the ends of filamentous cells. each can develop into the original organism
In Actinomycetes
Some _________ can repoduce by fragmentation into smaller motile fragments that glide away from parental strans (in aquatic environments)
Cyanobacteria
(giant Bacteria) in surgeonfish, “give birth” to multiple prokaryotes which emerge from a dead mother cell
Epulopiscium
Arrangement of Prokaryotic cells, result from 2 aspects of division during binary fission
Planes in which cells divide
Separation of daughter cells
If they seprarate or stay attached
Separation of daughter cells
Pairs of cocci
Diplococci
Chain of cocci
Streptococci
Cocci divdies into 2 planes remaining attached
Tetrads
Cocci divides into 3 planes remaining attached, cuboidal packets
Sacrinae
Giant clusters, appearance of grape clusters
Staphylococci
_______ are less variable in their arrangement , division occurs across transverse plane. May exist __________________.
Bacilli
-Singly, as pairs or chains
______ whihc divide by snapping division may result in palisade or V-shaped arrangement
Bacilli
Defensive strategy against unfavorable conditions
1-vegetative cell —-> 1 Endospore, 1 endospre —-> vegetative
Endospores
Produced by gram-positive
Bacillus and clostridium
Location of ______ may be in different locations of cells
Endospores
Modern prokaryotic classifications, currently based on gentic relatedness of DNA, RNA (_____) , and protein sequences
rRNA
3 domains of modern prokaryotic classification
Archaea
Bacteria
Eukarya
Lack a Nucleus
Prokaryotes
Common features of Archaea
Lack true peptidoglycan
Cell memranes lipids have branched hydrocarbon
AUG codon codes for methionine (same as eukaryotes)
Other common features of Archaea
Reproduce by binary fission, bdding, or fragmentation
Most are cocci, bacilli, or spiral forms;Pleomorphic forms
Not Known to cause disease
3 phyla (based on rRNA
Crenarchaeota (thermophile)
Euryachaeta (methanogens)
Korachaeta
Neve found, only observed samples of rRna in environment
Korachaeta
Require extreme conditions to survive; temperature, pH, and/or SAlinity
Extremophiles
Prominent members of Extremophiles are
Thermophiles and halophiles
(most in phylum crenarchaeota) DNA, RNA, cytoplasmic membranes, and proteins do not function properly below 45 C
Thermophiles
Require temperatures over 80C
Hyperthermiophiles
Two representative genera in thermophiles _______ and ________
Geogemma
Pyrodictium
extremeophiles / Halophiles:( 1st 2)
Inhabit extremely saline habitats
-Depends on greater than 91% NaCl to maintain integrity of cell walls
Extremophiles / Halophiles (2nd 2 )
Many contain red or orange pigments which may protect from visible and UV light
-Most studies - Halobacterium and Salinarium
Largest group of archaea (phylum Euryarcheatoa)
Methanogens
Convert carbon dioxide, hydrogen gas and organic acids to methane (CH4) gas
Methanogens
COnvert organic waste in pond, lake, and ocean sediments to methane
Methanogens
some live in colons of animals; one of primry sources of environmental methane
Methanogens
Scienetists believe these organisms are similar to earliest bacteria
Deeply branching bacteria
Live in habitats similar to those though to exist on early earth ; autotropic
Deeply braching bacteria
Represent more primitive forms of bacteria
Deeply braching bacteria
Phototrophs that contain photosynthetic lamaellae
Phototropic bacteria
Phototrophs are divided into ___ groups based on their ________________________.
5
-Pigments and source of electrons for photosynthesis
blue-green bacteria(cyanobacteria -green sulfur bacteria -green nonsulfur bacteria -purple sulfur bacteria purple nonsulfur bacteria
phototrophs 5 groups
Gram negative phototrophs, have chlorophyll a and produce O2
Blue-green bacteria
have heteorcysts, which are specialized sacs which have enzymes for nitrogen fixation N2 —–> NH2
Blue-green bacteria
derive e- from H2S (deposit sulfur outside cells)
Green sulfur bacteria
Derive e- from carbonhydrates, organic acids
Green nonsulfur
Derive e- from H2S (deposits sulfur inside cells)
Purple sulfur bacteria
Derive e- from carbohydrates, organic acids
Purple nonsulfur bacteria
Bacteriochlorophylls anoxygens
Green sulfer bacteria
green nonsulfur bacteria
purple sulfur bacteria
purple nonsulfur bacteria
Bacteria with guanine and cytosine below 50% in their nucleicacid
Low G+C gram-positive bacteria
Rod-shaped, obligate anaerobes; important in medicine and industry
Clostridia
Facultative or obligate anaerobes; lack cell walls, however have sterols in membranes; small free-living cells
Mycoplasmas
Low G+C Gram positive bacteria
Bacillus Listeria Lactobacillus Streptococcus and enterococcus Staphylococcus
Endospre forming aerobes facultative anaerobes, many common in soil; gram-positive bacteria
Bacillus
Contaminates milk and meat products (reproduces even if refrigerated) survuves in phaocytes ; gram positive
Listeria
Grows in the body but rarely causes disease (mouth, stomach, intestines vagina); gram positive
Lactobacillus
Causes numerous disease (ex. Strep throat, impetigo, pneumonia) Gram positive
Streptococcus and enterococcus
One of the most common inhabitants of humans (skin) may numerous disease gram-positive
Staphylococus
High G+c gram-postive bacteria
Corynebacterium
Mycobacterium
Actinomycetes
Pleomorphic aerobes and facultative anaerobes; produces metachromatic granules (stain differently) High gram-Positve
Corynebacterium
Aerobic rods that sometimes form filaments; slow growth partly due to mycolic acid in its cell walls high gram positive
Mycobacterium
from branching filaments resembling fungi (include actinomyces and streptomyces) high gram positive
Actinomycetes