ch. 9 testing for covid using PCR

Question 1

1st step

Answer 1

nasal swab– cotton swab is inserted in the nostril to absorb secretions; can also be inserted in the throat

Question 2

2nd step

Answer 2

collect specimen; stored at 2-8C for up to 72hrs or proceed to rna extraction

Question 3

3rd step

Answer 3

purified rna extracted from deactivated virus

Question 4

4th step

Answer 4

Rt-qPCR

purified rna is reverse transcribed to cDNA and amplified by qpcr

Question 5

5th step

Answer 5

test results — positive SARS-COV2 patients cross the threshold line within 40 cycles — negative are below threshold