ch. 10 binomial nomenclature/ ELISA/ Western Blotting

Question 1

binomial nomenclature

Answer 1

used to accurately name organism

Question 2

describe the components in binomial nomenclature

Answer 2

Genus and specific epithet

Question 3

why is binomial nomenclature important

Answer 3

helps us to know if its a good or bad bacteria

Question 4

phylogenetic tree

Answer 4

grouping organisms according to common properties

Question 5

what are the common properties in the phylogenetic tree and what do the mutations serve as

Answer 5

fossils and genomes

mutations accumulated in their genomes serves as molecular clock– more mutations the longer time– older it is

Question 6

taxonomic hierarchy

Answer 6

domain
kingdom
phylum
class
order
family
genus
species

Did King Philip Come Over For Green Stamps

Question 7

ELISA

Answer 7

enzyme link immunosorbent assay

Question 8

How does ELISA work

Answer 8

known antibodies and unknown type of bacterium are added to a well; an antibody reaction identifies the bacteria

Question 9

Step 1 in ELISA

Answer 9

antibody is absorbed to well

Question 10

step 2 in ELISA

Answer 10

patient sample is added – complementary antigen binds to antibody

Question 11

step 3 in ELISA

Answer 11

enzyme linked antibody specific for test antigen is added and binds to antigen, forming sandwich

Question 12

step 4 in ELISA

Answer 12

enzymes substrate is added and reaction produces a product that causes a visible color change

Question 13

serological method and example

Answer 13

using an antibody to detect antigens on bacterial surfaces — ELISA

Question 14

Western Botting

Answer 14

identifies antibodies in a patients serum

detect a specific protein in a blood or tissue sample.

Question 15

step 1 process of western blotting

Answer 15

using gel electrophoresis to separate the sample’s proteins.

Question 16

step 2 western blotting

Answer 16

The separated proteins are transferred out of the gel to the surface of a membrane.

Question 17

step 3 western blotting

Answer 17

The membrane is exposed to an antibody specific to the target protein.

Question 18

last step in western blotting

Answer 18

Binding of the antibody is detected using a radioactive or chemical tag.