ch. 9 restriction enzyme making recombinant dna

Question 1

first step in restriction enzyme making recombinant dna

Answer 1

restriction enzyme cuts the double stranded dna at the recognition site

Question 2

second step

Answer 2

dna fragment with sticky ends is made

Question 3

3rd step

Answer 3

because dna is cut into 2 by same enzyme it can come join back together by base pairing

Question 4

4th step

Answer 4

dna comes back together either linear or circular

Question 5

final step

Answer 5

dna ligase splices sticky ends together between free 3’ to 5’ prime end