ch. 9 PCR Flashcards
1
Q
what is PCR used for
A
diagnose disease
clone dna for recombination
sequence dna
detect pathogens
amplify dna
2
Q
steps of pcr
A
denaturation, annealing, and extension occurs in 30 to 40 cycles takes over 4 hours
3
Q
describe the denaturation process
A
high temp of 94 C for 1 minute
to separate DNA strands
4
Q
describe annealing process
A
low temp of 54C for 45sec
so primers can bind to target sequence on single strand of dna by complementary base pairings
5
Q
describe extension process
A
temp of 72 C for 2 min
extension of new DNA strands from the primers and dna synthesis
