BMBL Flashcards
BMBL: For what is the IBC responsible?
Institutional biosafety committee
Responsible for biosafety issues stemming from recombinant DNA research. Institution may define additional roles.
BMBL: What is the definition of recombinant DNA?
Molecule constructed outside of a living cell with the capability to replicate in a living cell
BMBL: What is required to do work with recombinant DNA if the work is funded by the NIH or if it is performed at an institution funded by the NIH?
1) Approval by the NIH director, review by the NIH RAC (recomb DNA advisory committee) and approval by IBC
2) Or review by the NIH OBA (office of biotechnology activities) and approval by IBC
3) Or review by the RAC and approval by the IBC and IRB
4) Or approval by IBC prior to initiation of research
5) Or notification of the IBC simultaneous with initiation of work
BMBL: Match the descriptor to the BSC level I-III
A) Protects personnel and environment
B) Protects personnel, environment and material inside cabinet
C) Gas tight
1 - A, 2-B, 3-C
BMBL: What federal agency regulates the importation and interstate shipment of animal pathogens?
USDA
BMBL: Criteria for BSL 1-4 agents?
BSL-1: Typically not associated with disease in humans
BSL 2: Indigenous. Moderate-risk associated with disease of varying severity
BSL 3: Indigenous or exotic. Potential for respiratory transmission. Can cause serious or fatal disease.
BSL 4: Dangerous and exotic. High risk of life threatening disease. Aerosol transmission. No vx or treatment available.
BMBL: Facility entry requirements for BSL 2-4?
BSL-2: Doors should be self closing
BSL-3: Two self-closing doors
BSL-4: Secure, locked doors. Logbook required.
BMBL: PPE requirements in BSL 2-4?
BSL 2: Protective lab coat must be worn. Eye and face protection when splashes possible. Eye, face and resp. protection should be used in rooms with infected animals. Gloves must be worn.
BSL 3: Solid-front protective lab clothing. Decon prior to laundering. Eye, face, resp protection required in rooms with infected animals.
BSL 4: Street clothes removed in outer change room. Lab clothing must be used. Remove lab clothing in inner change room. Decon before laundering.
BMBL: Requirements for removing biological materials from BSL-4 lab?
Non-breakable, sealed primary container that is in a non-breakable, sealed secondary container. Must go through dunk tank, chemical fumigation or decon shower.
BMBL: Autoclave requirements for BSL 3 and 4?
BSL 3: Preferably within lab. Should be within facility.
BSL 4: Must be provided. Double door. Can’t open outer door until it has gone through decon cycle.
BMBL: Class I BSC
Personnel protection, no product protection
Inward air flow >75 linear feet per minute (LFM)
Must be hard connected to building exhaust if toxic vapors will be used
BMBL: Class II BSC
Personnel and product protection
Type A1 >75 lfm, type A2 and type Bs >100 lfm
Type As recirculate air through HEPA filter back into room or be thimble or canopy connected to building exhaust and type Bs must be discharged directly to outdoors via hard connection
BMBL: What types of BSCs can be used for volatile toxic chemicals? What about minute/trace amounts of nonvolatile toxic chemicals?
Volatile - Class I and class II type B2 exhausted outside
Minute nonvolatile - Class II type B1 and A2 canopy exhausted
BMBL: Can flammable chemicals be used in class II type 1 BSCs?
No! Recirculation of air causes vapor buildup –> Fire hazard
BMBL: What type of class II BCS is total exhaust?
Class II type B2
Expensive to operate
Exhausts 1200 cubic ft/min
