Biotechnology Flashcards
Hybridization
Individuals w/ desirable traits are mated to produce offspring w/ a COMBINATION of both traits (Horse+Donkey=Mule, Liger….sterile) type of selective breeding
Evolution
Change in population (species) overtime, we can cause it
Pros and cons of selective breeding
Pros=favorable characteristics
Cons=results not guarenteed (variation) selective breeding for certain traits may cause problems w other traits, sterility
Inbreeding/risk
Selective breeding incest used when #s of available organisms are small; recessive disorders expressed (like hemophilia) sterility
GMO
Genetically modified organism w/ desirable traits, may disturb food chain if accidentally released into wild (salmon)
Transgenic
Contains DNA from other species (GMO)
GMO risks
Creation of new allergens, fear of unknown effects on future generations
GM viruses pro/con
Pro=make vaccines
Con=used in biological warfare
DNA technology
Branch of biotech where scientists alter genomes of organisms @ molecular level (DNA codes for proteins tht determine our traits)
Vectors
A way of getting genes from 1 organism to another (bacterial plasmids, viruses)
Plasmids
Circular bacterial chromosomes (human=x) splice genes into them and use them as vectors
2 main reasons for using bacteria/plasmid
HGH (human growth hormone) or insulin (diabetes)
Genetic engineering Recombinant DNA
Cut desired gene at palindrome and combine with plasmid (gene splicing). Insert new recombinant DNA into cell of another organism. Clones recombinant DNA when cell undergoes mitosis
Restriction enzyme
Cuts plasmid and the gene being added
Genome
Complete set of an organisms genetic material
What codes/ doesn’t code for proteins in organisms?
Genes code, parts that don’t are called non-coding regions
Linkage map
Shows the relative locations of genes on a chromosome
Human Genome Project
Completely map human genome by sequencing the order of nucleotides and distinguishing regions that code/non-code
Importance of human genome project
- Show EVOLUTIONARY RELATIONSHIPS by comparing genes of diff species
- Identifying genes help in diagnosing, treating, and preventing DISEASE
Gene therapy
Putting correct gene into our cells, NOT inherited (injected in body cell not sex cell)
Gene therapy challenges
Vector makes person sick, gene delivered to many/the right kinds of cells, delivered to right part of the cells genome, gene needs to be expressed/turned on
Hybrid vs GMO
- Hybrid=sex cells, selective breeding (cows)
- GMO=no sex, transgenic, only hereditary if gene implanted early (plasmid, glow pigs)
- BOTH ARTIFICIAL
Artificial selection (Selective Breeding)
Individuals with desirable traits are mated to produce offspring w/ those traits (DOGS)
Why do we fingerprint?
To identify ppl (CSI), paternity/maternity testing (who da babydaddy??) to see how closely 2 species are related