Bacillus, Trueperella, Listeria Flashcards
Listeria monocytogenes
Causative agent of human listeriosis
Agent of septicemic disease affecting rabbits and GPs
Most important in animals (birds and humans)
L. monocytogenes characteristics
Gram + intracellular bacteria, motile, non-spore forming, facultative anaerobe, rod shaped
Catalase and L- rammnose + and oxidase -
Resistant to NaCl
Beta hemolysin
L. monocytogenes expresses it
Causes RBC destruction
L. monocytogenes serotypes
Serotyped according to somatic (O) and flagellar (H) AGs
More than 14
Listeriosis
Sickens pregnant women and newborns, adults >65, immunocompromised
Facial and trigeminal n. Damage (facial paralysis and salivation)
Listeriosis food safety alert (CDC)
NY and PA found in Ice Cream House brand (Ice cream cups)
Enoki mushrooms
Deli meat and cheese
Listeriosis transmission
Eating contaminated food
Person to person (sexual contact)
Mother to child (pregnancy or childbirth)
Where has L. monocytogenes isolated from?
Meat or milk of goats, sheep, cattle, pig, chicken, quail, partridge, ostrich, buffaloes, fish
Veggies, seafoods
How long does listeriosis survive?
@ 5C survives for 13 (milk), 16 (brain), 12 (feces and silage) years
Listeria illness
Invasive: everywhere
Noninvasive: intestine
Symptoms start within 2 weeks after eating food
Listeria symptoms
Headache, stiff neck, confusion, meningitis, loss of balance, convulsions in pregnant women
Abortion, stillbirth, premature birth, septicemia
Circling disease (sheep)
Septicemic (horses and pigs), encephalitic (circling), abortion form
Causes encephalitis, repeat breeding endometriosis and abortion
Conjunctivitis in ruminants, mastitis in cattle
Listeriolysin O
Most significant virulence factor
Responsible for B-hemolysis on erythrocytes and destruction of phagocytic cells
Internalins (inIA)
Membrane proteins promoting cellular uptake
Virulence factor for Listeria
Listeriolysin (LLO)
Mediates intracellular survival and escape from phagosomes
Virulence factor for Listeria
Phospholipases
Involved in lateral spread to adjacent cells
Virulence factor for Listeria
L. innocua
Rare and cause meningoencephalitis in sheep (circling)
Acute and frequently fatal
L. ivanovii
Causes abortion in sheep and goats
Late abortion after 7 months in cattle and 12 weeks in sheep