B1- microscopes + microscopy practical

Question 1

What are Light Microscopes?

Answer 1

light microscopes use light and lenses to form an image of a specimen and magnify it. Allow you to see individual and sub-cellular structures like the nuclei.

-Low magnification of up to 2000x
-Low resolving power (resolution) of below 0.3 micrometers
-lens made of glass

Question 2

What are Electron Microscopes?

Answer 2

-uses beams of electrons to view specimens
-high magnification of up to 1,000,000x
-high resolving power of up to 0.0001 micrometers
-lens made of electromagnets

Question 3

What is the equation for Magnification?

Answer 3

Magnification = Image Size/Actual Size

Question 4

What is the method of preparing a cover slide?

Answer 4

1-Add a drop of water to a side
2-Place the specimen in the water
3-Place the edge of the coverslip on the slide so that it touches the edge of the water.
4-Slowly lower the coverslip to prevent forming and tapping air bubbles as they will obstruct the view.

Question 5

How do you use a microscope (Light)?

Answer 5

1- clip the slide onto the stage
2-click lowest power objective lens (the one that produces the least magnification)
3-use coarse adjustment knob to just below the objective lens
4-look down at the eyepiece, use coarse knob to move the stage downward until image is roughly in focus
5-adjust focus with fine adjustment knob until image clear
6-if you need a greater magnification swap to a higher power objective lens + refocus