Autosomas Flashcards

1
Q

Clasificación con base a su tamaño y posición del centromero

A

Metacentrico (centrada)
Submetacentrico ( 1 brazo mayor que otro)
Acrocentrico ( brazo corto extremadamente pequeño)
Telocentrico (1 solo brazo)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Grupos cr

A

A - G

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Grupo cr A

A

cr 1- 3
+ grandes
1 y 3 metacentricos / 2 submetacentrico

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Grupo cr B

A

cr 4 y 5
grandes
Submetacentricos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Grupo cr C

A

6 - 12 y X
medianos
submetracentricos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Grupo cr D

A

13 - 15
medianos
acrocentricos con satélites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Grupo cr E

A

16 - 18
pequeños
16 metacentrico / 17 y 18 submetacentricos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Grupo cr F

A

19 y 20
pequeños
metacentricos

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Grupo cr G

A

21,22 y Y
pequeños
acrocentricos
21 y 22 co satelites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Identificación por bandeo (Bandas G)

A

70’s
Ricas A y T
1. Desnaturalización tripsina
1. Tinción Giemsa (oscuras +G / blancas -G)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Identificación por bandeo (Bandas Q)

A

Tinción fluorecente (quinacrina)
Unión principalmente a A y T
Aprecio con luz UV

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Identificación por bandeo (Bandas C)

A
  1. Desnaturalizo hidróxido barico
  2. Tinción Giemsa
    Identifica heterocromatina constitutiva / región centromérica
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Identificación por bandeo (Bandas T)

A

Identificación de bandas cercanas a telomero
1. Desnaturalizo con temp.
2. Tinción con Giemsa

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Identificación por bandeo (Bandas R / Q-neg)

A

Inverso a G
Desnaturalización (quito rico en T y A) con temp. y sal → Giemsa
También se puede tinción con cromocina reg. ricas en G y C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

FISH (fluorescence in site hibridization)

A

Sonda de DNA con fluorecencia (primer)
pintado de:
- Cromosomas específicos
- Centromeros
- Reg. telomericas
Microdeleciones (perdida) cromosomicas
Cariotipo multicolor

How well did you know this?
1
Not at all
2
3
4
5
Perfectly