Autosomas Flashcards

1
Q

Clasificación con base a su tamaño y posición del centromero

A

Metacentrico (centrada)
Submetacentrico ( 1 brazo mayor que otro)
Acrocentrico ( brazo corto extremadamente pequeño)
Telocentrico (1 solo brazo)

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2
Q

Grupos cr

A

A - G

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2
Q

Grupo cr A

A

cr 1- 3
+ grandes
1 y 3 metacentricos / 2 submetacentrico

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3
Q

Grupo cr B

A

cr 4 y 5
grandes
Submetacentricos

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4
Q

Grupo cr C

A

6 - 12 y X
medianos
submetracentricos

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5
Q

Grupo cr D

A

13 - 15
medianos
acrocentricos con satélites

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6
Q

Grupo cr E

A

16 - 18
pequeños
16 metacentrico / 17 y 18 submetacentricos

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7
Q

Grupo cr F

A

19 y 20
pequeños
metacentricos

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8
Q

Grupo cr G

A

21,22 y Y
pequeños
acrocentricos
21 y 22 co satelites

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9
Q

Identificación por bandeo (Bandas G)

A

Ricas A y T
1. Desnaturalización tripsina
1. Tinción Giemsa (oscuras +G / blancas -G)

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10
Q

Identificación por bandeo (Bandas Q)

A

Tinción fluorecente (quinacrina)
Unión principalmente a A y T
Aprecio con luz UV

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11
Q

Identificación por bandeo (Bandas C)

A
  1. Desnaturalizo hidróxido barico
  2. Tinción Giemsa
    Identifica heterocromatina constitutiva / región centromérica
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12
Q

Identificación por bandeo (Bandas T)

A

Identificación de bandas cercanas a telomero
1. Desnaturalizo con temp.
2. Tinción con Giemsa

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13
Q

Identificación por bandeo (Bandas R / Q-neg)

A

Inverso a G
Desnaturalización con temp. y sal → Giemsa
También se puede tinción con cromocina reg. ricas en G y C

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14
Q

FISH (fluorescence in site hibridization)

A

Cariotipo multicolor
Para ver cositas específicas

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