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Portage Microbiology > 4.3 Obtaining a Pure Culture > Flashcards

4.3 Obtaining a Pure Culture Flashcards

1
Q

What is plating?

A

process of spreading a bacterial culture onto a petri dish filled with agar

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2
Q

How can plating be accomplished (what tools)?

A

sterile loop
sterile swab
sterilized wire loop

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3
Q

What is the collection method for skin/accessible membranes?

A

sterile swab brushed across the surface

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4
Q

What is the collection method for blood?

A

needle aspiration from the vein

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5
Q

What is the collection method for CSF?

A

needle aspiration from subarachnoid space of spinal column

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6
Q

What is the collection method for stomach?

A

intubation

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7
Q

What is the collection method for urine?

A

aseptic collection = catheter
clean catch = midstream

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8
Q

What is the collection method for the lungs?

A

sputum

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9
Q

What is the collection method for diseased tissue?

A

surgical removal via biopsy

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10
Q

What is the primary advantage of plating bacterial samples onto agar?

A

cells are held in place and support the visualization of colonies that are formed

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11
Q

What is used to help microbiologistst isolate a microbe on an agar plate?

A

quadrant streak

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12
Q

What is a quadrant streak also known as?

A

phase-dilution

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13
Q

What is the quadrant streak?

A

generate an individual colony so that a single bacterial sample can be picked from the plate

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14
Q

What is a four phase dilution gradient?

A

sample is rpead into four regions where each new region is perpendicular to the previous region

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15
Q

What region of a four phase dilution gradient will contain the highest concentration of microbial growth?

A

phase one, first quadrant

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16
Q

What is important regarding a sterile loop in the creation of a four phase dilution gradient?

A

new sterile loop needed for each quadrant

17
Q

What occurs once samples are appropriately streaked onto the media and lids are placed back on?

A

inverted to prevent any potential contaminants from settling onto the surface of the agar

18
Q

What is the temperature and length of time that the agar plates are left in the incubator for?

A

37C
12-24 hours

Portage Microbiology (53 decks)

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