4.2 Agar Plates

Question 1

What are the two forms that growth media comes in?

Answer 1

solid
liquid

Question 2

What is a general description of solid growth media?

Answer 2

liquid growth media that has had a hardening agent added

Question 3

Where are solid growth media typically contained in?

Answer 3

sterile petri dish

Question 4

What is agar?

Answer 4

solid growth media that has an added polysaccharide derived from seaweed (algae) extracts

Question 5

What are colonies?

Answer 5

microbes that have grown and appear as individual isolated dots

Question 6

What is a lawn?

Answer 6

when the microbe has grown to cover the entire plate

Question 7

What is LB agar?

Answer 7

multi-purpose media capable of growing a wide variety of microorganisms

Question 8

What color are LB agar plates?

Answer 8

pale yellow

Question 9

What type of media is LB agar?

Answer 9

non-differential and non-selective

Question 10

What is LB agar commonly used to grow?

Answer 10

E. coli

Question 11

What is TSA or TSAYE?

Answer 11

trypticase soy agar

Question 12

What is the function of TSA/TSAYE?

Answer 12

multi-purpose media that can grow a wide variety of microorganisms

Question 13

What color are TSA/TSAYE plates?

Answer 13

pale yellow

Question 14

What type of media are TSA/TSAYE plates?

Answer 14

non-selective and non-differential

Question 15

What are BAP plates?

Answer 15

blood agar plates

Question 16

What is the composition of BAP plates?

Answer 16

derivative of TSA plates where mammalian blood is added to the plate at 5-10% concentration

Question 17

What type of mammalian blood is typically used for BAP plates?

Answer 17

sheep

Question 18

What color are BAP plates?

Answer 18

red

Question 19

What type of media are BAP plates?

Answer 19

enriched, non-selective, differential media

Question 20

What is the purpose of the red blood cells in BAP plates?

Answer 20

promote the growth of fastidious microorganisms