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Portage Microbiology > 4.2 Agar Plates Part 2 > Flashcards

4.2 Agar Plates Part 2 Flashcards

1
Q

What is the function of the differential part of BAP plates?

A

detect hemolytic activity

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2
Q

What is hemolysis?

A

breakdown of red blood cells

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3
Q

What are the categories of hemolysis?

A

alpha
beta
gamma

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4
Q

How do microbes capable of alpha hemolysis appear on a BAP and why?

A

greenish-brown color colonies due to the incomplete lysis of red blood cells

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5
Q

What is beta hemolysis?

A

ability of a microbe to completely lyse red blood cells

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6
Q

How do BAPs appear with microbes capable of beta hemolysis?

A

lysis presents as a distinct zone of clearing around the growing colony

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7
Q

What is gamma hemolysis?

A

absence or lack of hemolytic activity

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8
Q

What is the appearance of the colonies that are classified as gamma hemolysis?

A

white/tan in color growing on the red background of the unaffected blood agar plate

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9
Q

What color is a columbia CNA agar?

A

red

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10
Q

What type of media is a columbia CNA agar?

A

enriched, selective, and differential media

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11
Q

What does the media of columbia CNA agar contain?

A

anitmocrobial agents colistin and nalidixic acid

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12
Q

What is the purpose of the columbia CNA agar?

A

suppress the growth of gram negative bacteria and used to isolate gram positive microbes

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13
Q

What is the columbia CNA agar enriched with?

A

blood

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14
Q

What is a cholocate agar also known as?

A

CHOC or chocolate blood agar (CBA)

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15
Q

What type of media is a cholocate agar?

A

enriched, nonselective, nondifferential media

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16
Q

What is a chocolate agar a derivative of?

A

blood agar plate

17
Q

What do chocolate agar plates contain?

A

red blood cells that have been lysed by heat

18
Q

What gives the chocolate agar plate its color?

A

the lysed red blood cells

19
Q

What is the purpose of the lysed red blood cells in a chocolate agar?

A

release various growth factors into the media required for the cultivation of fastidious, pathogenic bacteria

20
Q

What are examples of bacteria you can grow on a chocolate agar?

A

gram negative Haemophilus influenzae and neisseria meningitis

Portage Microbiology (53 decks)

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