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Portage Microbiology > 4.2 Agar Plates Part 4 > Flashcards

4.2 Agar Plates Part 4 Flashcards

1
Q

How do non-pathogenic strains of E coli appear on SMAC plates?

A

red colonies; acidic conditions

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2
Q

How do pathogenic strains of E coli appear on SMAC plates?

A

white colonies; neutral to basic conditions

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3
Q

What is an EMB agar?

A

eosin methylene blue agar

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4
Q

What color is an EMB?

A

red color

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5
Q

What type of media is an EMB?

A

selective and differential

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6
Q

How are microorganisms differentiated in EMB?

A

ability to ferment lactose present in the media

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7
Q

What does the media contain in EMB?

A

eosin and methylene blue

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8
Q

How do non-fermenting microbes appear on an EMB?

A

white/tan colonies

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9
Q

How do lactose-fermenting microbes appear on an EMB?

A

dark purple or black colonies on the surface of the agar

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10
Q

What do E coli look like on an EMB?

A

metallic green shade

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11
Q

What is an MSA agar?

A

mannitol salt agar

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12
Q

What color is an MSA agar?

A

red

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13
Q

What type of media is an MSA agar?

A

selective and differential

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14
Q

What is the function of an MSA agar?

A

selective for Gram positive bacteria and can differentiate members of the Stahylcocci family

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15
Q

What does the media for MSA contain?

A

high concentration of sodium chloride
mannitol
pH indicator dye phenol red

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16
Q

What is the purpose of the sodium chloride in an MSA?

A

prevents the growth of certain microorganisms

17
Q

What is the purpose of the mannitol and pH indicator dye in the MSA?

A

differentiate pathogenic from non-pathogenic strains of Staph

18
Q

What is the pathogenic strain of Staph?

A

staph aureus

19
Q

Which type if Staph can ferment the mannitol in an MSA?

A

pathogenic strain

20
Q

What does the MSA with pathogenic staph look like?

A

lowers the pH changing the dye color from red to yellow

Portage Microbiology (53 decks)

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