4.1 Microbial Growth and Control

Question 1

What is growth media?

Answer 1

liquid or solid formulated to support microbial growth

Question 2

What does growth media contain?

Answer 2

essential nutrients to provide the microbe with a source of carbon and energy

Question 3

What is the normal nutrient composition of growth media?

Answer 3

sugars
amino acids
vitamins

Question 4

What is growth media with nutrients normally referred to as?

Answer 4

nutrient broth

Question 5

What is the most common nutrient broth?

Answer 5

LB media

Question 6

What does LB media stand for?

Answer 6

lysogeny broth

Question 7

What is LB media?

Answer 7

nutritionally rich liquid known for its ability to grow a vast array of microbes

Question 8

What are the two general classifications of specialized media?

Answer 8

selective
differential

Question 9

What is a selective media?

Answer 9

allows for only the growth of certain microbes and by extension, restricts the growth of all others

Question 10

What are some methods to making a selective media?

Answer 10

limiting amount of nutrients
varying degrees of pH
various chemical additives such as antibiotics

Question 11

Where is selective media often used?

Answer 11

medical laboratories for cultivation of human pathogens

Question 12

What is an example of a human pathogen that uses selective media?

Answer 12

Neisseria meningitides

Question 13

What is differential media?

Answer 13

distinguishes between two, often related, microbes

Question 14

What is an example of bacteria that would use differential media?

Answer 14

E. coli and Salmonella are both gram negative so needs differential media

Question 15

What is an enriched media?

Answer 15

used to grow fastidious microorganisms

Question 16

What is a fastidious microorganisms?

Answer 16

organisms with complex growth requirements such that is absent will not grow