W7- Lecture 35.4- Histology- Microscopy & ELISA

Question 1

can you label a microscope ?

Answer 1

did you get 
the nose piece 
eye piece 
the objective 
stage
condenser
base

Question 2

explain how a bight field microscope/ Kohler illumination manipulates light to be able to see it a specimen

Answer 2

light source is collected by a condenser which focus’ the light onto the specimen
the objective lens collects the light and directs it into the eyepiece able for us to see

Question 3

what is phase contrast microscopy and why do we use it ?

Answer 3

uses light phase amplitude differences within microscopic objects
allows viewing of unstained specimens

Question 4

what is Normarski/ differential interference contrast DIC

when is this used ?

Answer 4

uses polarised light with prisms to add a 3d shadow by adding additional contrast
used to investigate living cell non invasively and in real time

Question 5

what is confocal microscopy ?

advantages/ disadvantages

Answer 5

uses a laser to excite florescent dye in a sample
then with this extremely precise image a computer creates a 3d reconstruction

advantages-
excellent resolutio for thick samples
collects light from a single focal point

disadvantages
photobleaching/ phototoxicity
increased sensitivity to noise
the method is labour intensive