VIROLOGY - VIRUS DETECTION METHODS

Question 1

what is the readily available technique for detecting virus for characteristic viral inclusions

Answer 1

cytology and histology

Question 2

this virus detection method involves the morphologic study of cells or tissues

Answer 2

cytology and histology

Question 3

are intracellular structures formed by aggregates of virus or viral components in an infected cell of abnormal accumulations of cellular materials resulting from virus induced metabolic disruption

Answer 3

viral inclusion

Question 4

are aggregates of cells fused to form one large cell with multiple nuclei

Answer 4

syncytial cells

Question 5

rabies virus inclusions in brain are called

Answer 5

negri bodies

Question 6

this virus detection method is most helpful for detecting viruses that do not grow readily in cell culture and works best if the titer or virus is at least 10^6-10^7 particles per milliliter

Answer 6

Electron microscopy

Question 7

this allows visualization of virus particles present in numbers too small for easy direct detection

Answer 7

immuno-electron microscopy

Question 8

the addition of specific antiserum to the test suspension causes the virus particles to form antibody-bound aggregates, which are more easily detected than single virus particles

this virus detection method refers to

Answer 8

immuno-EM

Question 9

in the clinical virology lab, this method of detection is most useful for detecting gastroenteritis viruses that cannot be detected by other methods (e.g. astroviruses) and encephalitis-causing viruses that are undetectable with cell culture (GSV, neasles, Jc polyomavirus )

Answer 9

Electron microscopy

Question 10

when there’s a newly recognized viral syndrome, it can be rapidly recognized through _____ by identifying characteristic viral morphology in an infected tissue

Answer 10

EM - electron microscopy