LEC2 - Specimen Collection Flashcards

1
Q

in specimen collection, we must Document the following

A

✓ Patient Identifiers
✓ Sample Identifiers
✓ Location and type
✓ Date and Time of Collection
✓ Relevant clinical information including recent and current antimicrobial the

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2
Q

At least how many mlof body fluids

A

2 mL

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3
Q

Transport and process within ____

A

2 hours

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4
Q

Store at how many Celsius if there is a delay in processing

A

4°C

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5
Q

we must store the sample in 4*C Except ____, store at 35
37°C

A

Blood, Bone Marrow, CSF and sterile tissues

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6
Q
  • Pus and discharge samples are collected using
A

cotton wool swab

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7
Q

only an experienced ___ collects eye

A

ophthalmologist

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8
Q

Intraocular fluid (___ and ____)

A

vitreous and aqueous

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9
Q

if the sample is insufficient - priority to ___

A

culture

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10
Q

Ear Swab and Scraping, who collects the sample

A

physician

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11
Q

Ear Swab and Scraping

Skin scrapings from the ____ preferred

A

external auditory canal

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12
Q

Deeper ear infections - ___ or experienced physician
should use a speculum to draw specimen.

A

ENT surgeon

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13
Q

collect exudate, pus or debris from the
lesion or inflamed area within the oral cavity

A

sterile oral swab

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14
Q

in oral swab,

___ aids visualization

A

tongue depressor/spatula

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15
Q

Low and High Vaginal Swabs

  • ___ be cleared prior to sample collection
A

mucous discharge

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16
Q

Low and High Vaginal Swabs

low vaginal swab (LVS) - who collects the sample

A

gynecologist or by the patient
herself

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17
Q

Low and High Vaginal Swabs

who collects high vaginal swab (HVS)

A

gynecologist - speculum
and swab stick

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18
Q

Adults - two samples (_____ ml each) - aerobic and anaerobic blood culture bottle

A

10 ml

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19
Q
  • Children - a single sample (___) - pediatric blood culture bottle
A

4-10 ml

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20
Q

Lumbar Puncture

  • a single sample (____ ml CSF) - trained physician
A

1-10

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21
Q

Urine

____ ml midstream urine in a sterile container - first morning urination.

A

10-20 ml

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22
Q

in a catheterized urine sample, what clamp is used

A

clamp hub of foley’s catheter distally

23
Q

in a catheterized urine sample, we clean the hub using

A

pyodine and 70% alcohol

24
Q

24-hour urine collection and Foley’s catheter tip samples are ___

A

not acceptable

25
Q

Surgical collection and punch biopsies are for

A

skin lesions

26
Q

In biopsy

grains - collect aseptically for ___ and ____

A

smear and culture

27
Q

____ be added to the tissue - prevent
drying

A

Sterile normal saline

28
Q

Exudates, Pus and Drainage

are collected by whom

A
  • physician or staff nurse - open wound, ulcer or abscess
29
Q

Exudates, Pus and Drainage

send several swab samples for
preparation of __

A

fungal smear
and inoculation of culture plates.

30
Q

in an open wound we * Do not swab __areas

A

dry crusted

31
Q

Ulcer
* Prior to specimen collection remove all debris - wash
with __

A

normal saline

32
Q

Ulcer

biopsy specimen - we collect in what area

A

edge of the wound or aspirate with a needle

33
Q

Abscess

  • Clean skin using _____ -
A

70% alcohol

34
Q

abscess

palpate point of ___

A

highest fluctuation

35
Q

abscess, we aspirate fluid from the ___

A

most fluctuant point

36
Q

Abscess

obtain sample from ___ of lesion and abscess wall

A

base

37
Q

Sputum
* first morning expectorated sample (optimal) with the following
instructions:

A
  • Clean mouth with several rinses of sterile saline or water.
  • Cough out 2-5 ml of sputum (not saliva) in a wide-mouthed
    sterile container
38
Q

how many ml of sputum should the patient cough out

A

2-5 ml

39
Q

sputum

dry cough - perform ____

A

sputum induction with hypertonic saline
nebulization

40
Q

Acceptability of sputum with a Gram-stained smear (pus cells
_____, epithelial cells _____)

A

> 25/LPF; <10/HPF

41
Q

Tracheal Aspira

collected through an ___

A

endotracheal tub

42
Q

Tracheal Aspirate

subject to the same limitations as ___

A

sputum specimens

43
Q

lower respiratory tract sample without the need for
bronchoscopy.

A

Non-Directed Bronchoalveolar Lavage

44
Q

suction catheter down the endotracheal
tube until resistance is met.

A

Non-Directed Bronchoalveolar Lavage

45
Q

Inject an aliquot of sterile saline - aspirate at least 1 ml of
secretions.

A

Non-Directed Bronchoalveolar Lavage

46
Q

Skin Scrapings
* Clean with ___alcohol

A

70%

47
Q

skin scales - collected using

A

sterile petri dish or similar wide-mouthed container
or clean, dry piece of paper folded securely with Scotch tape labelled properly.

48
Q

Skin Scraping

not yield sufficient material-

A

swab or Scotch tape could be pressed
on the lesion

49
Q

nails are * Examine for ___

A

damaged, discolored, brittle or dystrophic area

50
Q

Hair and Scalp
* _____ - helpful in selecting specimens

A

Wood’s light

51
Q

Hair and Scalp

how to collect hair

A

Pluck broken or lusterless hairs from periphery of lesion / Scrape
scalp from edge of hair loss area

52
Q

_in Piedra infections -how do we collect the sample

A

cut infected hair with scissors.

53
Q
A