LEC2 - Specimen Collection Flashcards
in specimen collection, we must Document the following
✓ Patient Identifiers
✓ Sample Identifiers
✓ Location and type
✓ Date and Time of Collection
✓ Relevant clinical information including recent and current antimicrobial the
At least how many mlof body fluids
2 mL
Transport and process within ____
2 hours
Store at how many Celsius if there is a delay in processing
4°C
we must store the sample in 4*C Except ____, store at 35
37°C
Blood, Bone Marrow, CSF and sterile tissues
- Pus and discharge samples are collected using
cotton wool swab
only an experienced ___ collects eye
ophthalmologist
Intraocular fluid (___ and ____)
vitreous and aqueous
if the sample is insufficient - priority to ___
culture
Ear Swab and Scraping, who collects the sample
physician
Ear Swab and Scraping
Skin scrapings from the ____ preferred
external auditory canal
Deeper ear infections - ___ or experienced physician
should use a speculum to draw specimen.
ENT surgeon
collect exudate, pus or debris from the
lesion or inflamed area within the oral cavity
sterile oral swab
in oral swab,
___ aids visualization
tongue depressor/spatula
Low and High Vaginal Swabs
- ___ be cleared prior to sample collection
mucous discharge
Low and High Vaginal Swabs
low vaginal swab (LVS) - who collects the sample
gynecologist or by the patient
herself
Low and High Vaginal Swabs
who collects high vaginal swab (HVS)
gynecologist - speculum
and swab stick
Adults - two samples (_____ ml each) - aerobic and anaerobic blood culture bottle
10 ml
- Children - a single sample (___) - pediatric blood culture bottle
4-10 ml
Lumbar Puncture
- a single sample (____ ml CSF) - trained physician
1-10
Urine
____ ml midstream urine in a sterile container - first morning urination.
10-20 ml
in a catheterized urine sample, what clamp is used
clamp hub of foley’s catheter distally
in a catheterized urine sample, we clean the hub using
pyodine and 70% alcohol
24-hour urine collection and Foley’s catheter tip samples are ___
not acceptable
Surgical collection and punch biopsies are for
skin lesions
In biopsy
grains - collect aseptically for ___ and ____
smear and culture
____ be added to the tissue - prevent
drying
Sterile normal saline
Exudates, Pus and Drainage
are collected by whom
- physician or staff nurse - open wound, ulcer or abscess
Exudates, Pus and Drainage
send several swab samples for
preparation of __
fungal smear
and inoculation of culture plates.
in an open wound we * Do not swab __areas
dry crusted
Ulcer
* Prior to specimen collection remove all debris - wash
with __
normal saline
Ulcer
biopsy specimen - we collect in what area
edge of the wound or aspirate with a needle
Abscess
- Clean skin using _____ -
70% alcohol
abscess
palpate point of ___
highest fluctuation
abscess, we aspirate fluid from the ___
most fluctuant point
Abscess
obtain sample from ___ of lesion and abscess wall
base
Sputum
* first morning expectorated sample (optimal) with the following
instructions:
- Clean mouth with several rinses of sterile saline or water.
- Cough out 2-5 ml of sputum (not saliva) in a wide-mouthed
sterile container
how many ml of sputum should the patient cough out
2-5 ml
sputum
dry cough - perform ____
sputum induction with hypertonic saline
nebulization
Acceptability of sputum with a Gram-stained smear (pus cells
_____, epithelial cells _____)
> 25/LPF; <10/HPF
Tracheal Aspira
collected through an ___
endotracheal tub
Tracheal Aspirate
subject to the same limitations as ___
sputum specimens
lower respiratory tract sample without the need for
bronchoscopy.
Non-Directed Bronchoalveolar Lavage
suction catheter down the endotracheal
tube until resistance is met.
Non-Directed Bronchoalveolar Lavage
Inject an aliquot of sterile saline - aspirate at least 1 ml of
secretions.
Non-Directed Bronchoalveolar Lavage
Skin Scrapings
* Clean with ___alcohol
70%
skin scales - collected using
sterile petri dish or similar wide-mouthed container
or clean, dry piece of paper folded securely with Scotch tape labelled properly.
Skin Scraping
not yield sufficient material-
swab or Scotch tape could be pressed
on the lesion
nails are * Examine for ___
damaged, discolored, brittle or dystrophic area
Hair and Scalp
* _____ - helpful in selecting specimens
Wood’s light
Hair and Scalp
how to collect hair
Pluck broken or lusterless hairs from periphery of lesion / Scrape
scalp from edge of hair loss area
_in Piedra infections -how do we collect the sample
cut infected hair with scissors.
