VIROLOGY - specimen transport, storage, and processing

Question 2

ideally all specimens collected for detection of virus should be processed immediately

t or f

Answer 2

true

Question 3

culturing of virus is recommended in the past, however, it was considered impractical, why is that so?

Answer 3

due to potential biohazards, sophisticated processing steps, and necessary QA controls

Question 4

specimens for viral isolation is not allowed to sit at what temperature

Answer 4

room or higher temp , should be kept at 4*C and transported to the lab immediately

Question 5

if there will be a delay in testing, the specimen must be stored at what temp

Answer 5

refrigerated not frozen

Question 6

samples must be tested within __ hrs of collection

Answer 6

12-24 hrs

Question 7

for storage up to 5 days, we store the sample in what temp

Answer 7

4*C

Question 8

for storage up to 6 days or longer, we store the sample in what temp

Answer 8

-20C, Preferrably -70C

Question 9

pre-requirement for specimens subjected to freezing, ano need gawin before i freeze

Answer 9

dilute or emulsified in VTM

Question 10

specimens that uses unapproved (USFDA )analytes must be tranported at what temp at freeze what at temmp and time

Answer 10

transported at refrigerated temp
freeze at -70*C if delayed longer than 2-3 days.

Question 11

calcium alginate is not recommended if the test is for PCR, aside from that, what is the virus it inactivates during culture

Answer 11

HSV - not recommeded for culturing

Question 12

VTM has antimicrobials, what are the 2 drugs used traditionally for such purpose

Answer 12

peniccilin 500 units/ml
streptomycin 500-1000 ug/ml

Question 13

In VTM, if serum is added for protein source, what type of serum is recommended

Answer 13

fetal calf serum, becoz it less likely contains inhibitors (Antibodies )

Question 14

respiratory, rectal, and stool specimen is can be maintained at what successful and known VTM?

Answer 14

HBSS
modified stuart’s
leibovitz-emory

they all contains antimicrobials

Question 15

blood for viral culture must be transported at sterile tube with ACG and kept at what temp

Answer 15

refrigerated at 4*C

Question 16

In processing blood, if testing for virus specific IgM, it should be processed before freezing, explain why

Answer 16

IgM may produce insoluble aggregates upon thawing producing false negative results

Question 17

lip and genital specimen must be tested for __ using __

Answer 17

HSV using culture and molecular methods

Question 18

urine specimen is submitted usually for __ detection

Answer 18

CMV

Question 19

in north america, stool sample must be tested for __ specially for infants 5 y/o and below

Answer 19

rotavirus