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MYCV > MYCO - CULTURE MEDIA AND INCUBATION REQUIREMENTS, STAINS > Flashcards

MYCO - CULTURE MEDIA AND INCUBATION REQUIREMENTS, STAINS Flashcards

1
Q

purpose of cycloheximide

A

to prevent the overgrowth of slow growing fungi by more rapidly growing species

important to note that cycoheximide may also inhibitory to some fungi

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2
Q

inhibitory agar controls bacterial contamination more effectively than sabouraud dextrose agar

true or false

A

true

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3
Q

is often used as an inhibitory agent for the growth of contaminating bacteria

A

chloramphenicol

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4
Q

chloramphenicol is used to inhibit contaminating bbacteria, however, what fungi is being inhibited by this antibiotic

A

Nocardia and other aerobic actinomycetes

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5
Q

growth of dimorphic fungi is enhanced on enriched media such as ____

A

brain heart infusion (BHI) containing antibiotics and 5% to 10% sheep blood

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6
Q

esculin-based media containing chloramphenicol and gentamicin or birdseed agar may be used for the cultivation of

A

cryptococcus spp

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7
Q

Agar tends to dehydrate during
the extended incubation period required for fungal recovery,
but this problem can be minimized by using culture dishes
containing at least ____ mL of agar and placing them in a humidified incubator.

A

40 mL

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8
Q

this media is used for primary recover of saprobic and pathogenic fungi

A

BHI

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9
Q
A
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10
Q

this agar provides a rich medium for bacteria, yeast and pathogenic fungi

A

BHI

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11
Q

this agar provides a rich medium for , yeast and pathogenic fungi

A

BHI with antibiotic

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12
Q

this media is for primary recovery of pathogenic fungi exclusive of dermatophytes

A

BHI with antiobiotic - walang bacteria na maggogrow dito.

diba cutaneous ang dermatophytes, sa cutaenous maraming bacteria, so inhibit yan para fungi llang ang maa-isolate

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13
Q

this media is used for the recovery of fungi from blood

A

BHI biphasic blood culture bottles

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14
Q

the mode of action of this media enhances the recovery of yeasts in blood

A

BHI biphasic blood culture bottles

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15
Q

this medium is for the isolation and presumptive identification of yeast and filamentous fungi

yeast and mold

A

chromogenic agar

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16
Q

chromogen mix contains substrates that react with enzymes produced by different organisms that result in the production of characteristic color changes

A

chromogenic agar

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17
Q

this media is for primary recovery of dermatophytes; recommended as screening medium

A

dermatophyte test medium

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18
Q

mode of action or principle of dermatophytes test medium

A

dermatophytes produce alkaline metabolites, which raise the pH and change the medium from red to yellow

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19
Q

mode of action of inhibitory mold agar

A

examines plates for growth. Chloramphenicol inhibits bacterial growth

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20
Q

enhances the recovery of blastomycosis and histoplasma capsulatum from contaminated specimens

A

yeasts- extract phosphate agar

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21
Q

what are the media that is for primary recover of saprobic and pathogenic fungi

A

BHI
potato flake agar
sabauraud with BHI

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22
Q

what are the media that is for primary recover of dermatophytes

A

BHI with antibiotics
dermatophytes test medium
inhibitory mold agar
mycosel agar
yeast extract phosphate agar

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23
Q

this differential media is for the detection of ascospores in ascosporogenous yeasts

A

acetate ascospore agar

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24
Q

in this differential media, potassium acetate is necessary and yeast extract increases the sporulation of yeasts

A

acetate ascospore agar

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25
this differential media is used for the identifation of cryptococcus trichosporon rhodoturia
'C'h'R'is'T'ensen 's urea agar
26
principle of christensen's ahar
produces urease and a change in the pH
27
component of christensen's agar
2% urea phenol red
28
acetate ascospore agar components
potassium acetate yeast extract dextrose
29
this differential media is for identification of candida albicans by chlamydospore production
cornmeal agar with tween 80 and trypan blue
30
cornmeal agar with tween 80 and trypan blue's components
cornmeal tween 80 trypan blue
31
in the media cornmeal agar with tween 80 and trypan blue, which component enhances the production of chlamydospores
tween 80
32
in the media cornmeal agar with tween 80 and trypan blue, which component provides contrasting background for observing the morphologic features of the yeasts
trypan blue
33
this media is for the conversion of the dimorphic fungus 'blastomyces' from mold to yeast form
cottonseed conversion agar
34
media composition of cottonseed conversion agar
cottonseed meal glucose
35
cottonseed conversion agar allows conversion of blastomyces spp mold to yeast form within how many days
3 days
36
this media is for the differential identification of Aspergillus spp.
czapeks's agar
37
czapeks's agar's composition
sodium nitrate sucrose yeast extract
38
this media is for the identification of cryptococcus neoformans and cryptococcus gattii
niger seed agar (birdseed agar)
39
principle of identification of C. neoformans and gattii using niger seed agar
produce enzyme 'phenol oxidase', resulting in a brown pigment through metabolism of caffeic acid
40
this media is for the detection of nitrate reduction to confirm 'cryptococcus spp'
nitrate reduction medium
41
principle of nitrate reduction medIum
if the cryptococcus yeast produces nitrate reductase, a cherry red indicates a positive test result
42
a media for demonstration of pigment production by trichophyton rubrum
potato dextrose agar
43
principle of potato dextrose agar
carbohydrate and potato infusion promotes the growth of yeasts and molds, while the low ph partially inhibits bacterial growth
44
this media is for identification of microsporum audouinii
rice medium
45
rice medium composition
white rice extract polysorbate 80
46
a component of rice medium that will enhance chlamydospores formation by candida albicans
polysorbate 80
47
rice medium differentiates microsporum canis to audouinii, what's their differences
canis - grows well in rice medium and with yellow pigment (isipin mo nalang canis- canin - dilaw na kanin) audouinii - no growth in rice medium
48
this agar will differentiate ff cryptococcus trichophyton mentagrophytes from rubrum
urea agar
49
what's the use of urea in urea agar
provides nitrogen source for organisms producing urease. Urease releases ammonia, which increases the pH and is indicated by a color change from red to yellow
50
identification of yeasts by determining fermentation
yeast fermentation broth
51
principle of yeast fermentation broth
most yeasts produce acid, which is indicated by a change in the solution from purple to yellow as a positive fermenter
52
identification of yeasts by determining carbon hydrate assimilation
yeast nitrogen-base agar
53
cultures should be examined at least how many times weekly during incubation
3 times
54
cultures should be incubated at room temp or preferably at 30*C for 21-30 days before they are reported as negative true or false
truelalu
55
cycloheximide can partially or completely inhibit what fungi
Cryptococcus neoformans candida krusei and other candida spp trichosporon P. boydii Aspergillus nocardia and other aerobic actnomycetes
56
this method is used to clear the specimen to make fungi more readily visible
potassium hydroxide
57
in potassium hydroxide , if five minutes clearing is not enough, additional how many minutes is necessary
five to 10 mns
58
advantage of potassium hydroxide
rapid detection of fungal elements
59
disadvanatage of potassium hydroxide
requires experience because background artifacts are often confusing, clearing of some specimens may require an extended time
60
this stain is used for the examination of melanin pigment in fungal cell walls
masson-fontana stain
61
time required for staining specimen using masson-fontana stain
1 hr and 10 mns
62
advantage of masson-fontana stain
aids differentiation of melanin and hemosiderin pigments
63
this stain is used for the detection of dungi in histologic section
methenamine silver stain
64
methenamine silver staining time
1 hr
65
best stain for detecting fungal elements
methenamine silver stain
66
disadvanatage of methenamine silver stain
requires a specialized staining method that is not usually readily available in microbiology laboratories
67
this stain is useful in examination of secretions for malignant cells
papanicolau stain
68
papanicolau stain staining time
30 mns
69
advantage of papanicolau stain
cytotechnologist can detect fungal elements
70
disadvantage of papanicolau stain
fungal elements stain pink to blue
71
periodic acid schiff stainining time
20 mn; five mn additional if counter stain is used
72
advantage of periodic acid schiff stain
stains fungal elements well; hyphae of molds and yeasts can be readily distinguished
73
disadvantage of periodic acid schiff stain
nocardia spp do not stain well
74
saline wet mounts time required
1 mn
75
advantage of saline wet mounts
quickly performed and cost effective
76
disadvantage of saline wet mounts
specimens must be fresh; not all specimens are visible with this preparation
77
wright's stain is used in
bone marrow or peripheral blood smears sample
78
wright's stain staining time
7 mn
79
advantage of wright's stain
detects histoplasma capsulatum and cryptococcus spp
80
disadvantage of wright's stain
most often used to detect H. capsulatum and cryptococcus spp in disseminated disease
81

MYCV (22 decks)

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