MIDTERM LEC TOPICS - ADENO, PARVO, PAPOVA Flashcards
1
Q
how many family of DNA viruses are there?
A
7 families
2
Q
how many family of RNA viruses are there?
A
14 families
3
Q
are adenovirus naked or with envelope
A
naked icosahedral
4
Q
type of DNA of adenoviridae
A
linear double stranded DNA
5
Q
size of adenoviridae
A
70-90 nm
6
Q
adenoviridae is first isolated from cultures of
A
human adenoids and tonsils in 1950
7
Q
the Only member of adenovirus cause human infection
A
Mastadenovirus
8
Q
how many serotypes of human adenoviruses have been described currently?
A
52 serotypes
9
Q
they infect wide range of host, animals, humans and birds
A
adenoviridae
10
Q
what disease are the most common clinical manifestation associated with adenovirus infection
A
respiratory and gastrointestinal disease
11
Q
adenoviridae infects infants and general population in how many percent?
A
18% of all acute acute diarrheal infection in infant
5% respiratory disease in general population
12
Q
what serotypes of adenovirus can cause gastroenteritis in infant and young children,
A
serotypes 40 and 41
13
Q
MOT of adenoviridae
A
aerosol/airborne
respiratory disease - respiratory secretions, stool, fomites
14
Q
what is the incubation period for respiratory disease caused by adenoviridae
A
2-14 days
15
Q
what are the common upper respiratory tract infections caused by adenovirus
A
colds, tonsilitis, pharyngitis, pharyngoconjunctival fever, and sometimes croup (viral infection of the larynx )
16
Q
complications of respiratory disease caused by adenoviridae to infants
or ano ung possible secondary infection nito sa infant if may respiratory disease
A
eye and conjunctivitis often accompany respiratory disease
otitis media - often a complication to respiratory disease
17
Q
describe the lower respiratory tract infection of adenoviridae
A
quite severe in children and the pneumonia is often fatal in infants and young children
18
Q
what is the unique feature of adenovirus that causes epidemics in the military
A
it can cause severe and acute respiratory disease , often resulting in considerable morbidity and mortality
19
Q
Adenoviruses can be detected from respiratory secretions or stool in cell culture using various epithelial cell lines such
A
A-549, Hep-2, and He-La cells –
20
Q
describe the cytopathic effect appearance of adenovirus
A
Grape like cluster cytopathic effect
21
Q
the viral capsid of adenoviridae has how many capsomere, hexons, and penton
A
252 capsomeres
240 hexons
12 pentons
22
Q
adenovirus
the genome consist of linear double stranded DNA with a molecule of approx. __ kilo base pairs
A
26-45
23
Q
adenoviridae is stable and viable for about _____
A
week at 37C but inactivated at 50C
24
Q
adenoviridae are resistant to
A
ether and bile salts
25
tropism of adenoviridae
nucleus of epithelial cells
26
genus under adenoviridae
mastadenovirus
aviadenovirus
27
which one of the genus of adenoviridae infects birds
aviadenovirus
28
85 serotypes of human adenovirus is divided into how many species
7 species (A to G )
29
commonly causes respiratory and gastrointestinal disease but is also associated with conjuctivitis, keratitis, and disseminated multi-organ disease for immunocompromised patients
adenovirus
30
MOT of adenoviridae
respiratort
feco oral
fomites
31
incubation period for adenoviridar
2-14 days for respiratory
3-10 days for gastro
32
the major cause of nonbacterial pharyngitis and tonsillitis, presenting as febrile common cold.
adenovirus
33
Adenovirus types what are associated with
pneumonia in adults resembling primary atypical pneumonia
3 and 7
34
ADENOVIRIDAE
the cause of an acute respiratory
disease (ARD) syndrome among military recruits.
serotypes 4,7,21
35
adenoviridae
it tends to occur in outbreaks, such
as at children’s summer camps
Pharyngoconjunctival fever:
36
Pharyngoconjunctival fever is associated with what serotypes
3, 7, 14
37
adenoviridae
a serious condition which
may appear as an epidemics, usually caused by type 8 and less often by types 19 and 37
Epidemic keratoconjunctivitis (EKC)
38
this condition caused by adenoviridae that occurs mainly in adults and is highly contagious
Epidemic keratoconjunctivitis (EKC)
39
acute follicular conjunctivitis caused by adenovirus are caused by which serotypes
3, 4, 11
40
which conjunctivitis is adenovirus similar with?
chlamydial conjunctivitis
41
Some fastidious adenoviruses can cause diarrheal disease
in children (serotypes __and ___)
40 and 41
42
systemic infection caused by adenovirus serotype 3 and 7
meningitis
43
test that can be done in nose and throat swab in VTM for detecction of adenovirus
virus culture
44
test that can be done in bronchoalveolar lavage fluid for the detection of adenovirus
pcr
45
test that can be done in nasopharyngeal aspirates for the detection of adenovirus
immunofluorescence - takes less than 2 hrs
46
test that can be done in conjunctival swab in virus from VTM in detection of adenovirus
culture and PCR
47
test that can be done in feces in the detection of adenovirus
PCR
rapid test devices
electron microscopy
48
is there antiviral chemotherapy available for adenovirus infection
No specific antiviral chemotherapy (supportive care only)
49
treatment and prevention for adenovirus infection in pools
For swimming poo-associated conjunctivitis-adequate levels of
CHLORINE in water
50
do we have vaccines available for adenovirus
yes but only for military recruits
Non-attenuated live Adenovirus types 4, 7, and 21 packaged in enteric capsules (military only
51
AAVs means
(Adenovirus Associated Viruses)
52
(Adenovirus Associated Viruses) are members of
parvoviridae
53
these are dependent with adenoviruses or herpes simplex virus to provide missing functions
(Adenovirus Associated Viruses)
54
* Smallest of the DNA viruses (~22nm
Parvoviridae
55
physical of parvoviridae
* Small, non-enveloped icosahedral capsid
Linear single stranded DNA genome
56
2 subfamilies of parvoviridae
densovirinae
parvovirinae
57
the subfamily of -parvoviridae that infects invertebrates (insects and crustaceans)
densovirinae
58
the subfamily of -parvoviridae that infects vertebrates
parvovirinae
59
3 important genus affecting humans of parvovirinae
dependoparvovirus
erythroparvovirus
bocaparvovirus
60
genus of parvovirinae that is the canine parvovirus
faudovirus
61
genus of parvovirinae that is a feline parvovirus
protoparvovirus
62
genus of parvovirinae that is a porcine parvovirus
tetraparvovirus
63
Characterized by their dependance on helper viruses such as
adenoviruses or herpesviruses for replication
Dependoparvovirus
64
Adeno-associated viruses (AAV)
* Can cause ___ illness
mild respiratory
65
* Can be used in gene-replacement therapy because of its low
immunogenicity and wide tissue tropism
Adeno-associated viruses (AAV)
66
Associated with a variety of respiratory infections including
bronchiolitis, pneumonia, and upper respiratory tract infections
which may become severe in young children
Human Bocavirus (HBoV)
67
Human Bocavirus (HBoV) mot
respiratory droplets
68
diagnosis` for Human Bocavirus (HBoV)
PCR, antigen detection, immunofluorescence, electron microscopy
69
Cell receptor: of parvovirus
P antigen
70
parvovirus B19
Cell receptor: P antigen found in the surface of
red blood cells, erythroid progenitors, vascular endothelium and fetal myocytes
71
parvovirus causes Infections such as
Infections causes no symptom or mild illness such as flu symptoms,
rashes and joint pains
72
slapped cheek” rash in children, and joint pains in adults is caused by
Parvovirus B19
73
parvovirus on Individual with weakened immune system can cause
low blood
count
74
parvovirus on Individual having a pregnancy can cause
miscarriage
75
parvovirus b19
* Virus spreads by
respiratory and oral secretion
76
parvovirus b19
Worldwide prevalence typically in __
children
77
Incubation period of parvovirus b19
12-18 days
78
At risk group of parvovirus b19
Pregnant women, immunocompromised patients and
those with hemolytic anemia
79
swelling of joint
Polyarthropathy Syndrome
80
Polyarthropathy Syndrome is caused by
parvovirus
81
Polyarthropathy Syndrome caused by parvovirus usually last____
1-3 weeks but can last for months
82
do Polyarthropathy Syndrome goes away without any long term problems?
yes
83
parvovirus infection
due to infection of red blood cell pre-cursors and
immune-mediated destruction
aplastic crisis
84
”slapped-cheek disease”
* Common in children aged 4-11 years
* May be associated with lymphadenopathy and joint symptoms
erythema infectiosum
85
excessive fluid build up in the fetus caused by parvovirus
hydrops fetalis
86
Diagnosis for parvovirus b19
Antibody Testing (Parvovirus IgM)-
pcr
pcr and antibody testing using cord blood
87
this diagnosis is for acute infection of parvovirus
antibody testing (parvovirus IgM)
88
this diagnosis is for acute infection of parvovirus especially immunocompromised and patients with aplastic crises
PCR
89
this diagnosis is for fetal infection caused by parvovirus
PCR and antibody testing using cord blood
90
treatment for parvovirus
supportive care
91
5th disease of parvovirus b19
erythema infectiosum
92
parvovirus b19 is under what genus
erythroparvovirus
93
PAPOVA IN PAPOVAVIRIDAE means
papilloma
polyoma
vacuolating
94
* Naked icosahedral capsid containing circular double stranded DN
PAPOVAVIRIDAE
95
Papillomaviridae will result to
Warts, condylomas, papillomas, cervical cancer
96
Polyomaviridae will result to
BK Virus, JC virus, Merkel Cell Polyomavirus,
Trichodysplasia spinulosa Polyomavirus
97
Papillomaviruses size
55 nm
98
papillomaviruses Can cause infections in
humans, dogs, cattle, monkeys and many
other species
99
Viral tropism of HPV
cutaneous/mucosal tissue
100
papillomaviruses is * Divided into more than ___serotype
200
101
in papilloma virumes, Envelope proteins E___ are transforming proteins associated with
initiating cancer
6 and E7
102
HPV high risk genotypes cause ce___ cancer
cervical, vulvar, vaginal, squamous cell
oropharynx, anal and penile
103
how many percent of all cervical cancers are caused by Hhuman papilloma virus
95%
104
in the 95% of the cervical cancers caused by HPV, the 70% is caused by
hpv 16 and 18
105
MOT of Papillomaviruses
Sexual, Vertical, Direct contact with infected material
106
Incubation period pf papillomaviruses
genital warts- 3 months after exposure,
cancer - several years from the acquisition of infection
107
Clinical Manifestations of papillomaviruses in skin
common warts
flat warts
epidermodysplasia verruciformis
108
common warts (verruca vulgaris ) is caused by what HPV
1, 2, 4,
109
Flat Warts (Verrucae plana) is caused by what HPV
HPV 3
110
Mucus Membrane clinical manifestation of papillomaviruses
genital wats
\laryngeal warts
111
1. Genital Warts is caused by what HPV
6 and 11
112
Laryngeal Warts is caused by what HPV
6 and 11
113
Carcinoma clinical manifestation of papillomaviruses
cervical cancer
other cancers such as cancer of squamous cell carcinoma found in penis, vulva, vagina, laryngeal carcinoma
114
Cervical cancer is caused by what HPV
16, 18, 31, 33, 35
115
Diagnosis for papillomaviruses
Histopathologic or cytologic examination of cutaneous
biopsy or cells, DNA probe assays, NAA
116
treatment for papillomaviruses
* Self-limiting and normally recede in 6-12 months
* Ablative treatments with podophyllin or liquid nitrogen
* Vaccines: Cervarix, Gardasil (HPV 6 and 11), and Gardasil-9 (HPV
31, 33, 45, 52, 58
117
Polyomavirus size
40-45 nm
118
characteristic of a Polyomavirus
icosahedral, naked, circular double-stranded DNA
119
polyomavirus is Believed to be transmitted through
respiratory or oral secretions
120
* Infections of these viruses usually occur during childhood and
appears to have little clinical significance.
Polyomavirus
121
polyomavirus
* Latency in __ can result in symptomatic
reactivation
kidney and B lymphocytes
122
MOT of polyomaviruses
Respiratory droplets
123
under polyomavirus
Had been isolated in urine from children with cystitis and evidence of
infection has been demonstrated in children with acute respiratory
illness
bk virus
124
BK virus,
Reactivation in renal transplant patients can result to
urethral strictures
125
bk virus, reactivation in bone marrow transplant patients can cause
hemorrhagic cystitis
126
Treatment for BK virus
cidofovir
127
Diagnosis for BK virus
PCR (blood and urine), Electron Microscopy of urinary sediments
128
under polyomavirus that is * Clinical importance in patients who are immunosuppressed due to
T-cell dysfunction in whom the virus reactivates to cause progressive
multifocal leukoencephalopathy (PML)
JC virus
129
diagnosis for JC virus
PCR (Brain biopsy or CSF), Serological Assays, Histological
examinations
130
MCPv integrates its DNA into the genome of
Merkel Cells
131
Diagnosis for merkel cell polyomavirus
Tumor biopsy, PCR | Treatment: Surgery, Immunotherapy
132
Growth of abnormal hair-like structures in the skin
Trichodysplasia Spinulosa Polyomavirus
133
Clinical presentation: red scaly patches on skin, itching or discomfort,
hair like growths
Trichodysplasia Spinulosa Polyomavirus
134
diagnosis of Trichodysplasia Spinulosa Polyomavirus
Skin biopsy | Treatment: Cidofovir cream, Corticosteriods,
Laser therapy
135
viruses under polyomaviruses
John Cunningham (JC), BK Virus, Merkel Cell
Polyomavirus, Trichodysplasia spinulosa polyomavirus
136
