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Immunoserology > TYPES OF PRECIPITATION REACTIONS > Flashcards

TYPES OF PRECIPITATION REACTIONS Flashcards

1
Q

What are the types of photometric techniques?

A

TURBIDIMETRY
NEPHELOMETRY

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2
Q

Aggregates are formed upon the mixture of antigens and antibodies, forming turbid or cloudy solutions

A

TURBIDIMETRY

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3
Q

Turbidimetry Measures the decrease of light intensity resulting from?

A

absorption, reflection or scatter.

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4
Q

measure the degree or rate of the turbidity within the solution

A

turbidimeter

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5
Q

Measures the light that is scattered from the incident light at varying angles (10°-90°)

A

NEPHELOMETRY

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6
Q

the rate of increased scattered light is measured immediately after the addition of the reagent antibody

A

Rate Nephelometry

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7
Q

What are the types of Passive immunodiffusion?

A

RADIAL IMMUNODIFFUSION (RID)
OUCHTERLONY DOUBLE DIFFUSION

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8
Q

Letting the Ag, Ab or both will diffuse within the medium where you will be going to test them

A

PASSIVE IMMUNODIFFUSION

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9
Q

Rate of diffusion is affected by?

A

Size of particles
Temperature
Gel viscosity
Amount of hydration

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10
Q

Ag-Ab complexes are allowed to precipitate and diffuse along the agar without any external influence (e.g. electricity)

A

Passive

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11
Q

Antigens are placed in wells carved out of an agarose gel medium

A

RADIAL IMMUNODIFFUSION (RID)

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11
Q

What is the medium that is used in RID?

A

Agarose gel medium

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12
Q

Holes in the medium where antigen will be administered

A

Wells

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13
Q

What indicates if there is a Ag-Ab complex precipitates are seen on the medium of RID?

A

“ring” or a circular area

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14
Q

What are the types of RID?

A

RID - MANCINI/ENDPOINT
RID - FAHEY/KINETIC

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15
Q

Antigen is allowed to diffuse at completion; no further diffusion or diameter increase is seen

A

RID - MANCINI/ENDPOINT

16
Q

RID - MANCINI/ENDPOINT Square of the diameter of the circle is equal to the _____________?

A

concentration of the antigen

17
Q

The thicker the circle = _______________

A

the higher the concentration of the antigen

18
Q

Measures the diameter of the precipitate at a specified time intervals

A

RID - FAHEY/KINETIC

19
Q

Considered to be more accurate than mancini method

A

RID - FAHEY/KINETIC

20
Q

RID-FAHEY KINETIC Diameter of circle is equal to the ___________________?

A

log of the concentration

21
Q

Both antigen and antibody will diffuse within the medium wherein it Detects for unknown antigens in samples through diffusion

A

OUCHTERLONY DOUBLE DIFFUSION

22
Q

What is created upon the diffuse and interaction of both antigens and antibodies?

A

Lines of identity or precipitin lines

23
Q

What is used to contain multivalent antibodies in ouchterlony double diffusion?

A

central well

24
Q

It means that the antigen present in the sample is the same as the standard. Precipitin line (Arched)

A

Serological Identity

25
Q

o Antigens that represent in the unknown does
not resemble the antigen present in the
standard
o Precipitin line (X cross)

A

Serological Nonidentity

26
Q

o Outlying line → SPUR
o The well that contain the unknown antigen is
somewhat the same to the standard antigen

A

Partial Identity

27
Q

Separation components through different electric charges

A

ELECTROPHORESIS

28
Q

Determine if proteins are positively or negatively charge

A

Amphotericity

29
Q

Application of electrical currents to diffusion precipitation reactions to increase sensitivity

A

IMMUNOELECTROPHORESIS

30
Q

What are the 2 phases of immuno electrophoresis?

A

separation
immunodiffusion

31
Q

Over production or underproduction of antibodies

A

Plasma cell Dyscrasia

32
Q

Who discovered the immunofixation electrophoresis?

A

Alper & Johnson

33
Q

antiserum is placed directly ON the surface on the medium, rather than in troughs

A

IMMUNOFIXATION ELECTROPHORESIS (IFE)

34
Q

What is the maon purpose of the IFE?

A

Supplement the finding in IEP

Immunoserology (21 decks)

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