LABELED IMMUNOASSAYS

Question 1

Testing a solution that is mixed together with sample and detectors

Answer 1

Homogenous

Question 2

Reduction of the colored product that are being produced

Answer 2

Competitive

Question 3

Analyte that we are looking for are competing for binding site within the test medium and competing with label markers

Answer 3

Competitive

Question 4

Abundant number of binding sites for both label markers and analyte.
The more product that are being produce is directly proportional to the amount or concentration of the analyte.

Answer 4

Non-competitive

Question 5

Immobilization of either antigen or antibody in the solid phase

Answer 5

Heterogenous

Question 6

Aka. Sandwich immunoassay

Answer 6

Capture Immunoassay

Question 7

A.k.a. Enzyme-linked Immunosorbent Assay (ELISA)

Answer 7

ENZYME IMMUNOASSAYS

Question 8

The most commonly used in Enzyme immunoassays

Answer 8

HRPO, (horseradish peroxidase)

Question 9

When choosing an enzyme as a label, they must have a high degree of _____________ and __________?

Answer 9

Stability
Specificity

Question 10

The measurement of light produced because of chemical reaction

Answer 10

CHEMILUMINESCENCE

Question 11

Production of light thru excitement of fluorophores / fluorochrome (Fluorescein isothiocyanate [FITC], rhodamine, Lucifer yellow VS);

Answer 11

IMMUNOFLUORESCENCE

Question 12

The antibody which will contain the label marker will directly interact with the antigen on the samples

Answer 12

DIRECT IMMUNOFLUORESCENT ASSAY (DFA)

Question 13

conjugated antibodies react with antigens

Answer 13

Fluorophore

Question 14

Most widely used for detecting diverse antibodies (autoantibodies)

Answer 14

INDIRECT IMMUNOFLUORESCENT ASSAY (IFA)

Question 15

Who introduced Radioimmunoassay?

Answer 15

Yalow and Berson (1959)