TYPES OF AGGLUTINATION REACTIONS Flashcards
Ideal for detecting UNKNOWN ANTIBODIES in a patient’s serum
DIRECT AGGLUTINATION
RBCs are used as indicators
HEMAGGLUTINATION
Semiquantitative analysis OF DIRECT AGGLUTINATION can be made through _______________?
Antibody titration
What are the examples of Agglutination tests?
Widal test and Blood typing
Detect antibodies for diagnosing typhoid fever
Widal Test
This uses a test kit containing antigens to salmonella bacteria
Widal Test
Direct agglutination, however what we are looking for is antigen (RBCin the indicator particle that will determine the blood type
Blood Typing
In blood typing, what are we looking for?
Antigen
Involves the conjugation of antigens with carrier particles (latex, gelatin, RBCs) that Detects UNKNOWN ANTIBODIES in samples.
INDIRECT/PASSIVE AGGLUTINATION
Used for detecting mostly IgM antibodies for the following infections:
o Group A Streptococcus (ASO Determination and Titer)
o Rotavirus
o CMV (Cytomegalovirus)
INDIRECT/PASSIVE AGGLUTINATION
Antibodies are conjugated to particles through adsorption that Detects UNKNOWN ANTIGENS in samples
REVERSE PASSIVE AGGLUTINATION
Antigens are stealing antigen binding sites from the antibodies
COMPETITIVE IMMUNOASSAY
what is the end result (positive) of competitive immunoassay?
absence of agglutination
Where can we use agglutination inhibition?
Drugs of abuse such as heroin and cocaine
Viral particle that binds to the surface of RBC
Spontaneous Agglutination