Treatment, Prevention, Control of Viral Diseases Flashcards
How do Neuraminidase inhibitors prevent the release of virus?
Prevent release of virus and spread of virus because hemagglutinin of virus is still bound/attached to the sialic acid containing receptors on surface of infected cell.
Dry heat sterilization method
Hot air oven, at least two hours at 160c
Antimicrobial Chemotherapy
Treatment of infectious diseases by drugs (chemical compounds) that are inhibitory or lethal to the pathogenic microbe
Resistance to Amantadine occurs by
Changes in amino acids that line the M2 channel, changes prevent the drug from plugging the channel
Differentiating Infected from Vaccinated Animal -DIVA
Vaccination with only live attenuated vaccines leads to a population of an antibody response that does not differ from the antibody response developed after natural infection.
Use of DIVA marker vaccines have a portion of the pathogen - less antigens than natrual strains
Antiviral Drugs
Class of medication used specifically for treating vial infections
Examples of Nucleoside Analog Reverse Transcriptase Inhibitors (NRTI’s)
Zidovudine (ZDV) / Azidothymidine (AZT)
Didanosine (ddI)
Targets for anti-retroviral therapy
Reverse transcriptase
Protease
Integrase
Fusion
Ways that live-attenuated virus vaccines are produced
Produced from naturally occuring attenuated viruses
Produced by attenuation of viruses by serial passage in cultured cells
Produced by attenuation of viruses by serial passage in heterologous host
Produced by attenuation of viruses by selection of cold-adapted mutants and reassortments
Types of non-replicating virus vaccines
Vaccines produced from inactivated whole virions
Vaccines produced from purifed native viral proteins
Internal Farm Biosecurity
Measures taken to combat spread of an infectious disease within the farm
Synthetic tricyclic amine of the adamantane family
Acts as both antiviral and anti-parkinson drug
Amantadine
Moist Heat Sterilization Method
Use of steam
Autoclave heated to 121c for at least 15 min at 15psi
T/F - Acyclovir is nontoxic to uninfected host cells
True
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Herpesvirus thymindine kinase and herpes virus DNA polymerase are not found in uninfected cells
Mechanism of ZDV / AZT
Resembles thymine deoxyribonucleotide triphosphate, reverse transcriptase cleaves two phosphates and inserts AZT monophosphate inot the cDNA that is being synthesized from viral RNA
Antisepsis
Application of a liquid antimicrobial chemical to skin or living tissue to inhibit or destroy microorganisms
Major membrane glycoproteins found on the surface of influenza virus
Neuraminidase (NA)
Hemagglutinin (HA)
Sterilization
Process that destroys or eliminates may or all pathogenic microorganisms, except bacterial spores on inanimate objects
Compared to antibiotics there are (more/less) antivirals available
Less
Mechanism of action of Amantadine
- Compound clog M2 channel and prevent it from pumping protons into the virion
- Viral RNAs remain bound to M1 and cannot enter nucleus
Non-Replicating Virus Vaccines
Vaccines produced from inactivated whole virions