Flaviviridae

Question 1

Classical Swine Fever virus is transmitted by

Answer 1

Oronasal route

Question 2

Infection with BVDV can result in what clinically different disease syndromes

Answer 2

Bovine Viral Diarrhea

Mucosal Disease

Question 3

Genera of Flaviviridae

Answer 3

Flavivirus

Pestivirus

Hepacivirus

Question 4

Diagnosis of BVDV

Answer 4

Clinical history and signs

Virus isolation in cell culture

Question 5

Diagnosis of Classical Swine Fever

Answer 5

Clinical Signs

Question 6

Characteristics of chronic mucosal disease

Answer 6

Skin ulcers, areas of hyperkeratosis and necrosis of crypt enterocytes

Question 7

West Nile Virus is maintained in

Answer 7

Mosquitos and Birds

Question 8

Characteristics of postnatal BVDV infection in non-pregnant cattle

Answer 8

Most common in young animals

Milid clinical disease

Biphasic fever, leukopenia, diarrhea, ulceration on lips/mouth/oral cavity, decreased milk production, thrombocytopenia

Question 10

Clinical sign of what disease pattern of BVDV

Answer 10

Chronic mucosal disease

Question 11

Diagnosis of West Nile Virus

Answer 11

IgM ELISA

Question 12

Cytopathic BVDV

Answer 12

Induce cytopathic effect in infected cells

Question 13

Non Cytopathic BVDV

Answer 13

Cause persistent infection

Question 14

Characteristics of BVBD infection in pregnant cows

Answer 14

Transplacental spread to fetus

Disease depends on fetus age:

• Early- embryonic death/reabsorption
• Before Immunological Competence - destructive fetal lesions, persistent postnatal infection, retarded growth “weak calf syndrome”, congenital defects
• Late- carriers of virus, seronegative.

Question 15

Clinical signs of West Nile Virus

Answer 15

Various Neurological Signs

Fever

Abnormal Gait

Head tremors

Muscle Fasciculation

Ataxia

Recumbance

Question 16

Mucosal Disease caused by BVDV

Answer 16

Sporadic disease occuring in persistently infected animals, usually as an aftermath of an epizootic infection

Question 17

Bovine Viral Diarrhea Virus (BVDV) belong to the genus

Answer 17

Pestivirus

Question 18

Pathogenesis of BVDV mucosal disease

Answer 18

Develop after infection with both non-cytopathic and cytopathic biotypes

Virus replicates throughout the GI tract and lymph nodes

Erosion or ulcers in mucosal epitheilum

Question 19

Clinical sign of what disease pattern of BVDV

Answer 19

Post natal infection in non pregnant cattle

Question 20

Bovine Viral Diarrhea

Answer 20

Acute disease, epizootic in nature in naive animals

Question 21

Primary replication site of Classical Swine Fever Virus

Answer 21

Tonsils

Question 22

Clinical signs of what Flavivirus

Answer 22

Classical Swine Fever Virus

Question 23

Lineages of West Nile Virus

Answer 23

WNV 1 and WNV 2

Question 24

Characteristics of persistant BVDV infection in calves and mucosal disease

Answer 24

Caused by non cytopathic virus

Failure to thrive

Mucosal disease develops when co-infected with cytopathic virus

Question 25

Secondary site of replication for Classical Swine Fever Virus

Answer 25

Lymphoid Tissue

Question 26

Clinical features of Classical Swine Fever Virus

Answer 26

Diffuse hyperemia, nervous system signs, lethargy, paralysis, tremors and convulsions

Question 27

Transmission of BVDV

Answer 27

Indirectly Directly from persistently infected animals

Question 28

BVDV mucosal disease is manifested by

Answer 28

Fever, anorexia, profuse watery diarrhea, erosion or ulcerative stomatitis

Question 29

Flaviviruses are inactivated by

Answer 29

Heat, common disinfectants, lipid solvents

Question 30

Clinical signs of what disease pattern of BVDV

Answer 30

Persistent infection in calves and mucosal disease

Question 31

Disease patterns arising for BVDV infection

Answer 31

Postnatal infection in non-pregnant cattle Infection in pregnant cows Persistent infection in calves and mucosal disease

Question 32

Replication of Flaviviruses occurs in the

Answer 32

Cytoplasm

Question 33

West Nile Virus is fatal in what species

Answer 33

Birds

Question 34

Pathogenesis of postnatal BVDV infection

Answer 34

Respiratory Replication in nasal mucosa and tonsils to lymph nodes and then to intestines

Question 35

West Nile Virus belongs to genus

Answer 35

Flavivirus

Question 36

Characterisistics of Flavivirus

Answer 36

Enveloped Single Stranded RNA Positive Sense Infectious RNA