Asfarviridae

Question 1

Only known DNA Arbovirus

Answer 1

African Swine Fever Virus

Question 2

Replication of Asfarviridae family occurs in the

Answer 2

Cytoplasm

__________________

Host cell nucleus is involved in early stage of DNA replication

Question 3

T/F: Humoral Immune response has substantial protective value against African Swine Fever Virus

Answer 3

False

__________________________

Infected pigs produce virus-specific antibodies, sera from infected pigs do not neutralize the virus, humoral immune response does not have substantial protective value

Question 4

T/F- There is a vaccine for African Swine Fever Virus

Answer 4

False

Question 5

Sites of african swine fever virus replication

Answer 5

Pharyngeal mucosa, tonsils

Endothelial cells

megakaryocytes

Macrophages

Question 6

Geographical distribution of African Swine Virus

Answer 6

Most of sub-saharan Africa

Italy

Question 7

Vector that African Swine Fever Virus can replicate in

Answer 7

Ornithodoros spp

________________________

Species of soft tick

Question 8

T/F- African Swine Fever is a notifiable diease

Answer 8

True

__________________

Infected and suspected infected farms must be placed in quarantine

Question 9

Clinical signs of chronic african swine fever virus infection

Answer 9

Emaciation and stunting

Swollen joints

Ulcers and reddened or raised necrotic skin foci

Pneumonia

Question 10

Effects of african swine fever virus are primarily

Answer 10

Hemorrhages and apoptosis

Question 11

Domestic cycle of African swine fever can be a result of

Answer 11

Bite from infected tick

Direct contact with infected animal

Indirect contact with fomite

Virus spread to new areas

Aerosol spread

Mechanical transmission through biting flies

Question 12

Clinical signs of peracute african swine fever virus infection

Answer 12

Pigs may die suddenly, or 1-3 days course of high fever, hyperpnea, hyperemia before death

Question 13

Clinical signs of acute african swine fever virus infection

Answer 13

High fever

Moderate anorexia

Erythema

Cyanotic skin blotching on the ears, tail, lower legs, hams

Respiratory disease

Recumbancy

Vomiting

Diarrhea

Abortion

Death

Question 14

General properties of Asfarviridae

Answer 14

Enveloped virions

Nucleocapsid core surrounded by internal lipid layers and complex icosahedral capsid

Genome is single molecule of linear double stranded DNA

Question 15

Hemadsorption caused by African Swine Fever virus is attributed to

Answer 15

Virus specific protein that appears on plasma membrane of infected cells during late infection

Question 16

Biological vectors of African Swine Fever virus

Answer 16

Ornithodorus sp

Question 17

African Swine Fever virus can survive in what type of conditions

Answer 17

30 days in pen

Several hours at pH 4 or pH 13

3 months in chilled carcass

2 years in 4C temps

Question 18

Domestic cycle of African Swine Fever virus

Answer 18

Primary outbreak of ASF in domestic swine

Question 19

Sylvatic Cycle of African Swine Fever Virus

Answer 19

Transmission of ASF between warthog and soft tick

Question 20

Diagnosis of African Swine Fever Virus

Answer 20

Virus isolation

Hemadsorption

Serology

PCR

Question 21

Host of African Swine Fever

Answer 21

All breeds and types of domestic pigs and european wild boar