Rhabdoviridae Flashcards
All Lyssaviruses cause what sort of disease?
Rabies or Rabies-like disease
Bovine Ephemeral Fever Virus belongs to what Rhabdovirus genus
Ephemorovirus
Clinical sign of what Rhabdovirus

Novirhabdovirus - Viral haemorrhagic Septicemia Virus
Exposure to Viral Hemorrhagic Septicemia virus is characterized by
Lethargy
Spiral Swimming Pattern
Darkened body color
Pale gills
Exophthalmia
Hemorrhage at base of fins
Clinical sign of what Rhabdovirus

Vesiculovirus
Rhabdovirus virions are released from the host cell by
budding
Vesicular Stomatitis Virus is transmitted by
Biting insects
Viral Hemorrhagic Septicemia Virus infection occurs at what water temperatures
4-14C, disappears at temperature >15C
What histiologic finding is diagnostic of Rabies Virus infection?
Negri bodies
Rhabdovirus protein that is an inner protein that facilitates virion budding - binds N and G proteins
M Protein
Clinical features of Vesicular Stomatitis Virus in cattle and horses
Excessive salivation and fever
Incubation period of Rabies Virus
14-90 days, even years
What type of sample should be collected for rabies virus detection
Tissue from at least two locations in the brain
Characteristics of excitative (fury) phase of rabies virus infection
Severe agitation and aggressiveness
Animal often bites any material
Rabid dogs develop typical high barking sound
Internal lesions associated with Viral Hemorrhagic Septicemia Virus infection
Hemorrhage in several organs
Liver is mottled and variably congested
Multifocal necrosis in several organs
Viral Hemorrhagic Septicemia Virus causes what type of disease in fish
Systemic infection
Gold standard laboratory diagnostic test for Rabies Virus
Fluorescent Antibody Test (FAT)
Four genera that make up Family Rhabdoviridae
Lyssavirus
Vesiculovirus
Ephemerovirus
Novirhabdovirus
Clinical Features of Bovine Ephemeral Fever Virus infection
Seasonal
Biphasic/Polyphasic Fever
Immediate drop in milk production
Cessation of rumination
Pathogenesis of Rabies Virus
- Virus replicates in the muscle tissue
- Accesses the peripheral nerves through sensory or motor nerves
- Binds to receptor for Ach at neuromuscular junction
- Infect the CNS
- Replicates extensively in limbic system
- Migrates to other organs - salivary glands
Protein that covers the surface of Rhabdoviruses
G Protein (Glycoprotein)
Rabies Virus is a member of what genus
Lyssavirus
Bovine Ephemeral Fever Virus is transmitted by
Arthropods
Infectious Hematopoietic Necrosis Virus belongs to what Rhabdovirus genus
Novirhabdovirus
Most of rabies is caused by
Rabid dog bites
Clinical feature of Vesicular Stomatitis Virus in swine
Lameness
Rhabdovirus protein that makes up the major capsid compent, the viral nucleocapid
N Protein
Rhabdovirus virions are (stable/unstable) in the envriontment
Relatively stable
Clinical sign of what Rhabdovirus

Vesiculovirus
Viral Hemorrhagic Septicemia Virus belongs to what Rhabdovirus genus
Novirhabdovirus
Clinical Phases of Rabies Virus infection
Prodromal Phase
Excitative (Fury) Phase
Paralytic (Dumb) Phase
RNA Viruses
Genome = Single Stranded, Linear, - Sense RNA
Enveloped

Rhabdoviruses
Characteristic of paralytic (dumb) phase of rabies virus infection
Characterized by the inability to swallow - photophobia
Leading to typical sign of foaming saliva around the mouth
Some animals may develop paralysis beginning at the hind extremities
Complete paralysis followed by death
Characteristics of the Prodromal Phase of Rabies Virus infection
Lasts 1-3 days with behavioral problems
Aggressiveness in tame animals
Daytime activities in nocturnal animals
No fear in humans in wild animals
Abnormalities in appetite
Rhabdovirus protein that is an RNA-dependent RNA polymerase - transcription and replication of viral RNA
L Protein
Characteristic microscopic lesions associated with what Rhabdovirus

Negri Bodies
Rabies Virus
Rhabdovirus protein that is a co-factor of the viral polymerase
P Protein
Vesicular Stomatitis Virus belongs to what Rhabdovirus genus
Vesiculovirus
Vesicular Stomatitis Virus isolation method of choice
RT-PCR
Rabies virus is secreted in what during fury phase
Saliva
Rhabdovirus virion properties
RNA Virus
Single Stranded
Linear
- sense RNA
Enveloped
Rod or Cone Shaped
Clinical features of Vesicular Stomatitis Virus in cattle and swine
Vesicls on tongue, oral mucosa, teats and coronary band
Clinical signs of infectious hematopoietic necrosis virus infection
Darkened body color
Lethargy
Pale gilles - anemia
Bilateral exophthalmia
Distention of abdomen - ascites
Hemorrhage at base of fin
Rhabdovirus replication occurs in the
cytoplasm
Vesicular Stomatitis Virus is (stable/unstable) in the environment
Stable
Gold standard diagnostic test for Bovine Ephemeral Fever Virus
Virus isolation in mosquito cell culture or suckling mouse brain
Rabies virus reservoirs in North America
Skunks
Raccoons
Foxes
Wolves
Clinical sign of what Rhabdovirus

Novirhabdovirus - Infectious Hematopietic Necrosis Virus
Bovine Ephemeral Fever Virus causes what disease
Three Day Stiff Sickness
Infectious Hematopoietic Necrosis virus infection occurs in what water temperature
Juvenile fish at water temperature 8-15C