Transcription And Translation Flashcards
Ribosome
Converts mRNA into a protein
By reading the information and converting nucleotides into a string of amino acids
The sequence of dna
Will determine the sequence of amino acids that make up the protein
Synthesis of a protein by a ribosome
Large ribosomal subunit: binds tRNA
Small ribosomal subunit: binds mRNA
Reads information, builds protein sending it where it needs to go
Difference between dna and rna
Stability:
DNA= very stable
RNA = falls apart quickly (because of the extra oxygen)
Structure:
DNA= double stranded
RNA = usually single stranded
Nucleotides:
DNA= ATGC
RNA= AUGC (uracil instead of thymine)
Base pairing :
DNA= GC/ AT
RNA= GC/ AU
Transcription is done by one enzyme
RNA polymerase
Creates rna
Information in the form of DNA
DNA takes on a larger structure called chromosome
The chromosome is essentially made up of little chapters, each which is called a gene.
Each chapter tells the story of making a protein.
Transcription in prokaryotic cell and eukaryotic cells
Prokaryotes: cytoplasm
Eukaryotic: nucleus
DNA template
Opposite of non template strand 5’-3’
Gene to be transcribed 3’-5’ by rna polymerase creating rna
subbing U for A
transcription bubble
Where base pairs are made
MRNA transcript
The message 5’-3’ direction
Always walking alone dna template transcribing from 3’-5’
***always on template strand !! Starting at 3 ‘ of template strand !!
Compare and contrast transcription in prokaryotic and eukaryotic cells with regards to RNA processing location where mRNA is created
accessibility to mRNA to the ribosomes upon the creation of the transcript
Prokaryotic‘s fast and repeated the translated in the cytoplasm with the ribosomes
Eukaryotic or slower entrance must be removed and spliced and is done in the nucleus allowing the seller to be flexible and create different proteins from the exact same sequence of information.
Ex: Immune system antibodies
tRNA
Transfer rna connects Info in mRNA to corresponding amino acid (dehydration synthesis)
Trna Anticodon: 3 letter sequence that will be associated with a particular amino acid
Ex: rna AAA will bring in anticodon UUU base pair creating the amino acid lysine ( reads from rna) adding it to peptide chain
Connects these two languages through the base pairing rules.
Start codon
Aug (met)
Stop codons
UAA UAG UGA
Start end
5’ untranslated region