Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Micro > Lab Final Exam > Flashcards

Lab Final Exam Flashcards

1
Q

What are the two methods for creating isolated colonies on a agar plate

A

T streak and quadrant streak

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

If you forget to sterilize your little between each section while streaking for isolation what would you predict your plate would look like after inoculation

A

Growth all over the plate with no isolated colonies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What type of media would you use if you were trying to determine the oxygen requirement for an organism

A

Fluid thioglycolate medium

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Bacteria growing and fluid thioglycolate broth growing half an inch below the surface

A

Microaerophile: Require oxygen to survive, require environments contain lower levels of oxygen than the atmosphere

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What growth patterns can you expect in thioglycolate medium?

A

Obligate aerobe: must use O2
Obligate anaerobe: must avoid o2
*Facultative anaerobes: can grow with it without O2
*Aerotolerant anaerobes: no metabolic o2 requirement but can tolerate.
Microaerophile: Require oxygen to survive but require environments that contain lower levels of oxygen in the atmosphere

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the name of the growth container used to check for Capnophilic growth

A

Candle jar

An aerobe jar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What are the four major thermal classifications of bacteria

A

Psychrophile: icy waters 10-20 degrees
Mesophile: body temp 37-45 degrees
Thermophiles: hot springs 60-80 degrees
Hyperthermophiles: ocean vents, above boiling

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Predict the outcome of growing a psychrophile at 65 Celsius and predict the outcome of going in Mesophile at 37 success

A

A psychrophile would not grow at 65°C

Mesophile at 37 will thrive and grow very well

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What are the three major classifications of bacteria based on the pH In which they grow

A

Acidophiles: below ph 2.0
Neutrophils: 6.5-7.5 ph
Alkaliphiles: 8.5-11ph

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Predict the outcome of growing an alkaphilic bacteria at a pH of 9.0 and predict the outcome of growing and acidophilic bacterium at PH9 point

A

Alkaliphiles at 9.0 would grow very well

Acidophiles at 9.0 would not grow at all

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the name of an organism that can grow at high salt concentration

A

Halophile

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What organisms in the lab can grow in high salt

A

Staphylococcus: aureus/ epidermis

MSA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What’s the purpose of heat fixing a slide

A

Kills the bacteria

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Chains of bacteria are known as

A

Strepto

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Which 2 bacteria morphologies can form chains

A

Coccus and bacillus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Irregular clusters of bacteria

A

Staphylo

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Which morphology can form irregular clusters

A

Coccus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

If you use a basic dies and perform a simple stain would you expect the cell to be the same color as the dye or would it be colorless and why

A

The same color as the dye, because the bacteria is slightly negative and so they are attracted to the positive chromophore of the basic dye

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

If you performed a negative stain on a bacterium which stain would you use and what would be the color bacteria and the color of the background

A

Eosin or nigrosin, bacteria would be colorless. Background would be dark/ black

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Gram stain

A

Primary: crystal violet
Counterstain: safranin
Positive: purple
Negative: pink

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Acid fast stain

A

Primary: carbol fuschin
Counterstain : methylene blue
Positive: pink
Negative blue

22
Q

Endospore stain

A

Primary: malachite green
Counterstain: safranin
Positive: green spores present
Negative: all pink

23
Q

What microscopy technique would you use to determine motility

A

Hanging drop method

24
Q

What is the function of a mordant and what is the mordant for Gram stain

A

Grams iodine, it is used to hold down the molecules of a stain onto a micro organism increasing staining ability

25
Q

Mannitol salt agar (MSA)

A

Halophile
Positive = turns yellow
Negative = stays red

26
Q

MacConkey agar

A

Used for gram negative lactose fermenting organisms.
Positive for lactose: pink (ex. E. Coli)
Non lactose: colorless/ yellow

27
Q

Eosin methylene blue (EMB) agar

A 
Selective: for gram negative 
Differential: for lactose and non lactose fermenting. 
Positive for lactose : dark black/ pink 
Negative lactose: colorless 
Metallic green: e. Coli
28
Q

Hektoen agar

A 
Salmonella and shigella
Uninoculaed: green 
Selective: gram negative 
Differential: lactose fermenting 
Yellow/orange: + lactose (e.coli)
Clear growth : shigella
Black: hydrogen sulfide production (salmonella)
29
Q

Xylose lysine deoxycholate (XLD) agar

A 
Salmonella and shigella
Selective: gram negative 
Yellow: e. Coli (lactose +)
Black: salmonella 
Red: shigella
30
Q

What color does this medium turn on an organism for men’s the sugar and the medium phenol red carbohydrate broth (PR glucose, lactose etc.)

A

Yellow= positive fermenting

31
Q

What color is the medium on an organism does not use the sugar

A

Red= negative fermenting

32
Q

What is the purpose of the Durham tube

A

Gas production

33
Q

Which 2 reagents are used to test for the VP results ? What color is a positive result?

A

Vp reagent A/ VP reagent B

Red= positive (small layer at top)

34
Q

What reagent would you use to determine if an organism is catalase positive or negative and what would each result look like?

A

Hydrogen peroxide
Bubbles +
No bubbles -

35
Q

Which test may require the addition of zinc powder to determine the final results.

A

Nitrate reductase media

36
Q

If you add zinc powder to the medium and it changes to red is this positive or negative result

A

Negative

37
Q

Is citrate medium broth or agar? What does this positive citrate result look like

A

Agar
positive =blue
Negative = green ( if green but has growth = positive)

38
Q

For decarboxylase test ADC, LDC, ODC What must you add to the top of the broth after inoculation? What color with the test turn if the organism can decarboxylate an amino acid

A

Mineral oil

Purple

39
Q

Which test requires you to add to a present iron chloride (FeCl3) ? if your test turns green is a positive or negative result?

A 
Phenylalanine deaminase (PDA)
Green = positive
40
Q

What color is a positive urease test?

A

Pink

41
Q

What color is a positive bike esculin test (BEA)?

A

Dark brown almost black = positive

Tan = negative

42
Q

What are the three things SIM medium can be used to determine

A

Motility, indole production , sulfur reduction

43
Q

If you add a Kovacs reagent to sim medium what would you be testing for and what color is a positive result

A

Indole production

Pink= positive

44
Q

If your sim is cloudy it is positive for

A

Motility

45
Q

If your sim is black

A

Sulfur reduction

46
Q

What is the difference between Alpha Beta Gamma hemolysis

A

Alpha is the partial destruction of our blood cells

beta is the complete destruction and gamma does not involve any breakdown

47
Q

What place is used to determine if a strain of bacteria is hemolytic or not

A

Blood agar plate (BAP)

48
Q

Which organisms would you be trying to confirm with coagulate test

A

Staphylococcus aureus

49
Q

Name and describe the method used to determine anabiotic susceptibility for bacterium

A

Disk diffusion method (the Kirby Bauer method)
Anabiotic desks diffuse into Mueller Hinton agar potentially inhibiting bacterial growth
* zone of inhibition indicates bacteria could not grow and presence of antibiotics
*resistance it will grow fine
*intermediate sort of kills it
*sensitive large zone of inhibition

50
Q

A small circular piece of DNA got the plasma can be put into a bacterium using which technique

A

Transformation

51
Q

What was the purpose of growing bacteria transformed with Pglo on plates that contained ampicillin

A

They are naturally ampicillin resistant so the ones that have picked up the plasmid are now going to grow on the ampicillin plate

52
Q

What is the purpose of growing bacteria transformed with P glow and plates that contained arabinose

A

If you add arabinose sugar they’re going to make that glowing fluorescent pro Tien

Micro (24 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions