DNA: Structure And Replication Flashcards
Genome
The entire set of genetic info within an organism
Ex: E.coli 4 million letters
Humans 3 billion letters
Chromosome
Structure made of dna that physically carries info
Ex: bacteria have 1
Humans have 23
Gene
Piece of dna that encodes for a product (typically protein)
Ex: E. Coli 4,000-5,000
Human 14,000-20,000
From biggest to smallest
Gene genome chromosome
Genome
Chromosome
Gene
Describe the central dogma of molecular biology
DNA >transcribed > rna >translated > protein
Diagram the structure of the DNA double helix including the following
identify the general composition of the four nucleotides found in DNA
Sugar phosphate backbone
Nitrogenous base (A, T, G, or C)
Draw out on white board
Explain the direction in which DNA is read and the chemical difference between the five prime and three prime sides of DNA
DNA as read from the left to the right five prime to three prime
five prime and has a sugar phosphate backbone the three prime and has the hydroxyl group and does dehydration synthesis
Describe the hydrogen bonds between the for nucleotides of DNA
A - T = two pairs of hydrogen bonds easier to split better place to start
G-C = Three hydrogen bonds much harder to split
Where are the base pairing rules for a DNA
A= T
G=C
Explain how the way in which information is included in starting dinner allows it to be copied dang cellular division
The origin were DNA replication begins as the replication fork by the enzyme that helicase vertical genetic transfer horizontal Gene transfer.
Always starts on the three prime side
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Diagram the replication fork including the following Helicase DNA gyrase DNA polymerase DNA ligase Leading strand Lagging strand Okazaki fragments The directions Leading and lagging strand are synthesized
Helicase: Split apart double helix by breaking the GC and AT base pairs allowing access to information
DNA gyrase: Also known as topsomerase release the tension and super coil DNA ahead of the Heli case
DNA polymerase: Read the parents Jan and synthesizes new complementary DNA strand continuously along the leading strand and non-continuously on the lagging strand using dehydration synthesis moving from 3 Prime to 5 Prime
DNA ligase : that is not between Okazaki fragments and connects the fragments on the lagging strand
Draw on whiteboard