Topic8 RNAi And siRNA Flashcards

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1
Q

What does RNAi stand for?

A

RNA interference

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2
Q

What does siRNA stand for?

A

Small interfering RNA

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3
Q

What is RNAi?
What does it do?

A

In eukaryotes and some prokaryotes
Translation of the mRNA produced from target genes can be inhibited by RNAi

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4
Q

How is translation of mRNA inhibited by RNAi?

A

This happens when mRNA molecule that’s already been transcribed gets destroyed before it’s translated

This is done by siRNA (small interfering RNA)

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5
Q

What does siRNA control?

A

siRNA control the translation control

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6
Q

What is the first thing that occurs in siRNA?

A

Enzymes cuts mRNA into siRNA

siRNA is double stranded and one strand of siRNA then combines with an another enzyme

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7
Q

How is siRNA formed?

A

siRNA is formed when an enzyme cuts mRNA into siRNA

So siRNA comes from mRNA

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8
Q

What is the structure of siRNA?

What is the second step in how does siRNA occur?

A

Structure of siRNA is double stranded and one strand of siRNA combines with another enzyme

Forms siRNA-enzyme complex and bind to complementary base pairing to another mRNA molecule as its singles stranded.

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9
Q

What is the structure of mRNA?

A

Short single stranded chain of polynucleotides

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10
Q

What is the last thing that occurs in how does siRNA occur?

A

When siRNA enzyme complex bind to another mRNA molecule.

Enzyme will cut up the mRNA so cannot be translated

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