The HLA System

Question 1

1. The HLA genes are located on which chromosome?
   a. 2
   b. 4
   c. 6
   d. 8

Answer 1

c. 6

Question 2

2. The majority of HLA antibodies belong to what immunoglobulin class?
   a. IgD
   b. IgE
   c. IgG
   d. IgM

Answer 2

c. IgG

Question 3

3. What is the test of choice for HLA antigen testing?
   a. Agglutination
   b. Molecular
   c. Cytotoxicity
   d. ELISA

Answer 3

b. Molecular

Question 4

4. Of the following diseases, which one has the highest relative risk in association with an HLA antigen?
   a. Ankylosing spondylitis
   b. Juvenile diabetes
   c. Narcolepsy
   d. Rheumatoid arthritis

Answer 4

a. Ankylosing spondylitis

Question 5

5. Why is HLA matching not feasible in cardiac transplantation?
   a. No HLAs are present on cardiac cells
   b. No donors ever have HLA antibodies
   c. Total ischemic time is too long
   d. Total ischemic time is too short

Answer 5

d. Total ischemic time is too short

Question 6

6. DR52 molecules are the product of which alleles?
   a. DRA and DRB1
   b. DRA and DRB3
   c. DRA and DRB4
   d. DRA and DRB5

Answer 6

b. DRA and DRB3

Question 7

7. What is the molecular technique that detects undefined
   alleles?
   a. Restriction fragment length polymorphism
   b. Sequence-specific primer typing
   c. Sequence-specific oligonucleotide typing
   d. Direct nucleotide sequencing

Answer 7

d. Direct nucleotide sequencing

Question 8

8. What represents the association of the alleles on the two
   C6 chromosomes as determined by family studies?
   a. Haplotype
   b. Genotype
   c. Phenotype
   d. Xenotype

Answer 8

a. Haplotype

Question 9

9. Which type of HSCT can be performed within a relatively short period of time?
   a. Matched unrelated
   b. Cord blood
   c. Autologous
   d. HLA haploidentical

Answer 9

d. HLA haploidentical

Question 10

10. The SAB describes the amount of bound antibody on
    each bead as:
    a. MCS
    b. MFI
    c. CDC
    d. AHG

Answer 10

b. MFI