Blood bank testing tech & automation

Question 1

1. The endpoint of the CAT test is detected by:
   a. Agglutination.
   b. Hemolysis.
   c. Precipitation.
   d. Attachment of indicator cells.

Answer 1

a. Agglutination.

Question 2

2. The endpoint of the SPRCA test is detected by:
   a. Agglutination.
   b. Hemolysis.
   c. Precipitation.
   d. Attachment of indicator cells.

Answer 2

d. Attachment of indicator cells.

Question 3

3. The endpoint of the solid-phase protein A assay is:
   a. Agglutination.
   b. Hemolysis.
   c. Precipitation.
   d. Attachment of cells to microwell.

Answer 3

d. Attachment of cells to microwell.

Question 4

4. Protein A captures antibodies by binding to the:
   a. Fab portion of immunoglobulin.
   b. Fc portion of immunoglobulin.
   c. Surface of test cells.
   d. Surface of indicator cells.

Answer 4

b. Fc portion of immunoglobulin.

Question 5

5. Mixed-field reactions can be observed in:
   a. Gel.
   b. SPRCA.
   c. Protein A technology.
   d. None of the automated technologies.

Answer 5

a. Gel.

Question 6

6. An advantage for both CAT and solid-phase technology is:
   a. No cell washing steps.
   b. Standardization.
   c. Use of IgG-coated control cells.
   d. Specialized equipment.

Answer 6

b. Standardization.

Question 7

7. A disadvantage for both CAT and solid-phase technology is:
   a. Decreased sensitivity.
   b. Inability to test hemolyzed, lipemic, or icteric samples.
   c. Inability to detect C3d complement–coated cells.
   d. Large sample requirement.

Answer 7

c. Inability to detect C3d complement–coated cells.

Question 8

8. A safety feature in the SPRCA test is:
   a. Air bubble barrier.
   b. Viscous barrier.
   c. Color change of the LISS.
   d. Use of IgG-coated control cells.

Answer 8

c. Color change of the LISS.